506  American  Pharmaceutical  Association.  {AloioberPi90im' 
Injected  hypodermically  with  5  c.c.  fluid  extract  ergot  assaying  0*25 
per  cent.  Cornutine  of  Keller  at  9.16  a.m.,  wattles  and  comb  red  and 
warm. 
At  9.37  a.m.    Comb  bluish  and  cooler,  wattles  beginning  to  blue. 
At  9.56  a.m.    Comb  very  blue  and  cold,  wattles  very  cold  and 
blue. 
At  11.36  a.m.  Comb  very  blue  and  cold,  wattles  very  cold  and 
blue. 
Experiment  V. — Cornutine  of  Keller  from  the  fluid  extract  used 
in  experiment  IV  injected  hypodermically  into  same  rooster  at  8.30 
a.m.,  wattles  and  comb  red  and  warm. 
At  9  a.m.    Comb  and  wattles  decidedly  blue  and  cool ;  bill  open. 
At  10  a.m.  Comb  and  wattles  very  blue  and  cold;  bill  open,  and 
panting. 
At  1  p.m.    Comb  and  wattles  still  very  blue  and  cold. 
Experiment  VI. — Filtrate  from  assay  of  experiment  V  neutralized 
and  injected  into  the  same  rooster,  his  comb  and  wattles  being  red 
and  warm  at  9.35  a.m. 
At  9.59  a.m.    Comb  and  wattles  still  red  and  warm. 
At  10.39  a.m.  Comb  pale  bluish  on  one  tip  and  warm  ;  wattles 
red  and  warm. 
At  12  m.  Comb  pale  bluish  on  one  tip  and  warm  ;  wattles  red 
and  warm. 
At  1.50  p.m.    Comb  and  wattles  still  red  and  warm. 
Experiment  VII. — Black  and  white  rooster,  4  pounds.  Comb  and 
wattles  red  and  warm.    Injected  5  c.c.  fluid  extract  ergot  at  TO. 5 3 
A.M. 
At  1 1. 10  a.m.    Comb  and  wattles  still  warm  and  red. 
At  1 1 .3 1  a.m.    Comb  bluish  at  tips,  comb  and  wattles  cooling. 
At  11.47  A-M-    Comb  bluer,  wattles  pale  and  cooler. 
At  12.09  P-M-    Comb  bluer,  wattles  pale,  almost  white  and  cool. 
At  12.55  P-M-    Comb  much  bluer  and  cold,  wattles  whitish  and 
cold. 
At  2  p.m.    Comb  very  blue  and  cold,  wattles  white  and  cold. 
At  5  p.m.    Comb  still  blue  at  tips  and  cool,  wattles  pink  and  cool. 
At  8.40  a.m.  next  morning.    Comb  and  wattles  red  and  warm. 
Experiment  VIII. — Black  rooster,  5  pounds.  Wattles  and  comb 
red  and  warm.  At  12  M.  injected  5  c.c.  Cornutine  ot  Keller  obtained 
from  fluid  extract  ergot  in  experiment  VII. 
