40(3 
A  nail/sis  of  Jlu.'^fdrd. 
f  Am.  Jour.  Pharnh. 
t     Ansiiist,  1882. 
sinapine  and  myroiiate  of  potash.  Subtracting  the  amount  of  tlie  lat- 
ter, the  difference  is  the  amount  of  sulphocyanide  of  sinapine. 
After  the  extraction  with  alcohol,  the  filter  contains  only  the  myro- 
sine  and  cellnlose,  together  with  a  little  coloring  matter.  The  alcohol 
is  allowed  to  evaporate  spontaneously,  and  then  the  myrosine  and  cel- 
lulose are  treated  in  the  cold  with  a  ^  per  cent,  soda  solution.  The 
solution  containing  the  myrosine  is  decanted  through  a  weighed  filter, 
and  the  residue  is  treated  again  in  the  same  manner.  By  this  treat- 
ment all  the  myrosine  is  obtained  in  solution.  The  cellulose  on  the 
filter  is  dried,  weighed,  ignited,  and  the  ash  weighed.  The  difference 
between  the  two  weights  gives  the  cellulose. 
The  solution  containing  the  myrosine  is  just  neutralized  with  dilute 
hydrochloric  acid,  and  about  50  cc.  of  Ritthausen^s  cupric  sulphate 
solution  added.  The  solution  is  then  exactly  neutralized  with  dilute 
soda,  and  the  heavy  green  precipitate  of  the  compound  of  copper  and 
myrosine  allowed  to  settle  to  the  bottom  of  the  beaker. 
The  precipitate  is  collected  on  a  weighed  filter  and  dried  at  110°. 
After  drying,  the  weight  of  the  precipitate  is  taken.  It  is  then 
ignited  and  the  ash  weighed,  the  difference  giving  the  total  amount  of 
myrosine. 
The  two  following  analyses  of  a  sample  of  brown  mustard  farina,, 
prepared  by  H.  K.  and  F.  B.  Thurber  &  Co.,  New  York,  were  made 
simultaneously.  Afterwards  a  third  analysis  was  made. on  the  same- 
sample. 
Analysis  of  Brown 
Mustard  Farina. 
1 
2 
3 
Moisture, 
.  6-78 
6-90 
6-82 
Myronate  of  iwtash, 
0-61 
0-61 
0.72 
Sulphocyanide  of  sinapine, 
.  10-97 
11-19 
11-21 
Myrosine, 
28-45 
28-70 
28-30 
Mustard  oil, 
.  29-22 
29-21 
29-19 
Cellulose  by  difference. 
20-24 
19-55 
20-06 
Ash, 
.  3-73 
3-84 
3-70 
100-00 
100-00 
100-00 
A  combustion  was  made  of  the  same  mustard,  and  the  nitrogen- 
determined.  The  sulphur  was  likewise  determined  by  fusing  with 
alkaline  carbonates  and  nitrates  and  precipitating  with  barium 
chloride. 
Nitrogen  —  5*337  per  cent.  Sulphur  =  1-489  per  cent.  Calcu- 
lating the  amounts  of  nitrogen  and  sulphur  iu  the  myronate  of  pot-. 
