Am\in£i?rm"}     Ike  Influence  of  Oxygen  on  Olive  Oil.  311 
Determinations  of  the  iodine  and  saponification  numbers  were 
then  made  with  oil  from  a  stoppered  blue  glass  bottle  from  which 
that  used  in  the  heating  experiment  above  described  had  been  ob- 
tained and  which  also  had  stood  three  weeks  at  room  temperature 
exposed  to  diffused  sunlight.  This  oil  had  not  lost  its  color  nor  had 
it  become  rancid.  On  opening  the  bottle  thirteen  months  afterward 
it  was  found  that  the  oil  had  not  lost  its  color  but  had  become 
strongly  rancid. 
Iodine  Saponification 
Number.  Number. 
Fresh  olive  oil  82  27  I92'95 
Number  of 
Determination. 
1.  Unheated  oil  which  had  stood  three  weeks  81 '40  194*  16 
2.  Unheated  oil  which  had  stood  three  weeks  80*73  Lost. 
Mean  '   81*06 
Oil  from  the  same  bottle  as  above,  which  had  stood  corked  with 
an  air  space  of  about  iOO  c.c.  above  the  surface  of  the  liquid,  gave 
the  following  results  : 
Iodine  Saponification 
Number.  Number. 
Fresh  olive  oil  82*27  T92'95 
Number  of 
Determination . 
r.    Unheated  oil  which  had  stood  thirteen 
months  76*03  181*17 
2.    Unheated  oil  which  had  stood  thirteen 
months  75"6i  181  "58 
Mean  75 '82         Mean  181*37 
Influence  of  an  atmosphere  consisting  solely  of  oxygen  as  affect- 
ing the  iodine  and  saponification  numbers  of  unsterilized  and 
sterilized  olive  oil  in  diffused  sunlight  and  in  darkness  on  standing 
thirteen  months  : — 
Iodine  Saponification 
Number.  Number. 
Fresh  olive  oil  82*27  192*95 
Number  of 
Determination. 
1.  Unsterilized,  in  diffused  sunlight  75 *o6  202*79 
2.  Same  specimen                                            75*30  201*33 
Mean 
Mean  2*02*06 
