312  The  Influence  of  Oxygen  on  Olive  Oil  {AmjJu°iy^9OTarm- 
1.  Unsterilized,  in  darkness1  60*15  246*30 
2.  Same  specimen  60*16  244  "47 
Mean  60*155       Mean  245-38 
1.  Sterilized,  in  diffused  sunlight  72*02  207*45 
2.  Same  specimen   7276  206*99 
Mean   72*39        Mean  207*22 
1.  Sterilized  and  kept  in  darkness   ....  73*71  i99"57 
2.  Same  specimen   73*05  200*95 
Mean   73*38       Mean  200*26 
All  of  the  above  specimens  of  oil  had  become  very  rancid.  That 
which  was  unsterilized  and  had  been  exposed  to  diffused  sunlight 
retained  a  slight  yellow,  tinged  with  green,  color.  The  unsterilized 
which  had  been  kept  in  the  dark  (seal  broken,  allowing  escape  of 
oxygen)  had  become  colorless.  The  sterilized  which  had  been 
exposed  to  diffused  sunlight  retained  a  slight  yellow,  tinged  with 
green,  color,  and  the  sterilized  which  had  been  kept  in  the  dark 
retained  a  yellow  color  with  a  decided  greenish  tint.  On  breaking 
the  sealed  tube  of  the  flasks  an  outward  pressure,  made  evident  by 
the  escape  of  gas,  was  observed. 
Influence  of  an  atmosphere  consisting  solely  of  nitrogen  as 
affecting  the  iodine  and  saponification  numbers  of  unsterilized  and 
sterilized  olive  oil  in  diffused  sunlight  and  in  darkness  on  standing 
thirteen  months: — 
Iodine  Saponification 
Number.  Number. 
Fresh  olive  oil  82*27  192*95 
Number  of 
Determination. 
1.  Unsterilized,  in  diffused  sunlight  ( 1st  flask)  81 -53  200*00 
2.  Same  specimen   82*00  200*50 
Mean  81*76  200*25 
1.  Unsterilized,  in  diffused  sunlight  (2d  flask)  83*39  I99*25 
2.  Same  specimen  83*72  I99'9I 
Mean   83*55       Mean  19958 
1  The  sealed  tube  of  the  flask  used  in  this  experiment  had  been  broken  at  an 
unknown  time  during  the  thirteen  months,  therefore  the  experiment  is  of  little 
value. 
