324  Lloyd  Reaction  as  Applied  to  Heroin.  {AmjJu°i^iSarm 
on  stirring,  except  in  the  mixture  containing  o.ooi  mgm.  of  each 
alkaloid,  which  produced  only  a  momentary  pale  pink-brown.  The 
same  play  of  colors  was  produced  in  mixtures  of  identical  quantities 
of  the  alkaloids  as  stated  between  8o°  and  900  C.  except  they  oc- 
curred and  disappeared  more  rapidly. 
Veratrin  in  the  same  proportions  as  employed  in  the  mixtures 
with  hydrastin,  when  treated  with  sulphuric  acid  alone,  or  with 
sulphuric  acid  and  potassium  dichromate,  produced  the  identical 
play  of  colors  as  that  yielded  by  the  mixtures,  except  in  the  quantity 
of  o-ooi  mgm.  of  veratrin,  which  gave  only  a  pale  yellow  color  with 
sulphuric  acid. 
As  small  a  quantity  as  0008  mgm.  of  veratrin  treated  with 
sulphuric  acid  produced  a  pale  pink  in  the  play  of  colors,  and  o-ooi 
mgm.  (1-65000  grain)  when  brought  to  a  temperature  between  8o° 
and  900  C.  yielded  a  pale  pink  color  in  a  few  seconds. 
From  the  results  of  these  experiments,  I  believe  it  justifiable  to 
conclude  that  veratrin  gives  no  characteristic  response  to  the  Lloyd 
reaction  for  alkaloids. 
The  royal  purple  color  obtained  by  Joseph  L.  Mayer6  with  a  mix- 
ture approximating  1  part  of  hydrastin  with  8  parts  of  veratrin 
(actual  weight  not  given),  is  no  doubt  the  same  color  which  I  have 
designated  as  violet-blue. 
In  applying  the  tests  to  the  mixtures  of  veratrin  and  to  veratrin 
alone,  the  same  general  plan  as  that  employed  in  making  the  tests 
for  heroin  was  followed. 
With  the  mixtures  containing  o-ooi  mgm.  of  hydrastin  to  0  001, 
0  006  and  0  009  mgm.  veratrin,  also  with  the  same  proportions  of 
veratrin  alone,  only  2  drops  of  sulphuric  acid  were  employed. 
Dr.  Wormley14  gives  the  delicacy,  under  ordinary  conditions,  of 
the  sulphuric-acid  test  for  veratrin  as  1-50000  grain  and  says  :  "  Much 
less,  if  collected  at  one  point,  and  touched  with  a  minute  drop  of 
the  warmed  acid,  will  yield  a  distinct  coloration."  The  delicacy  of 
this  test  for  veratrin,  at  a  temperature  between  8o°  and  900  C,  may 
be  represented  by  1-65000  grain  as  shown  in  my  experiments. 
DIONIN. 
A  few  preliminary  trials  of  the  Lloyd  reaction  with  another 
morphin  substitution  compound,  dionin  (ethyl  morphin  hydro- 
chloride 
