A^ctober59o?m'}     Examination  of  Lippia  Scaberrima.  451 
evaporated.  The  residue  left  by  the  latter,  when  extracted  with 
acidulated  water,  gave  no  reaction  with  alkaloidal  reagents. 
Quantitative  Estimation  of  the  lannin. — A  quantity  (17-3 
grammes)  of  the  finely  ground  herb  was  extracted  with  successive 
small  portions  of  boiling  water,  and  the  cold  filtered  liquid  diluted 
to  the  measure  of  500  c.c.  100  c.c.  of  this  liquid,  when  evaporated, 
and  the  residue  dried  in  a  water-oven  until  of  constant  weight,  yielded 
0-5402  gramme  of  extract ;  100  c.c.  of  the  same  liquid,  after  treatment 
with  hide  powder,  yielded  0  3498  gramme  of  extract.  The  amount  of 
substance  absorbed  by  the  hide  powder  was  therefore  o- 1904  gramme, 
which  would  correspond  to  5-5  per  cent,  of  tannic  matter  in  the  air- 
dried  herb. 
Extraction  with  Alcohol. — A  quantity  (7600  grammes)  of  the  dried 
herb  was  extracted  by  continuous  percolation  with  hot  alcohol, 
and  the  greater  portion  of  the  alcohol  removed.  The  resulting  thin 
extract,  which  had  a  dark  green  color  and  an  aromatic  odor,  was 
brought  into  a  large  flask  and  distilled  in  steam  until  oily  drops 
were  no  longer  visible  in  the  condenser.  There  then  remained  in  the 
distillation  flask  a  quantity  of  resinous  substance  {A)  and  a  dark- 
colored  aqueous  liquid  (B),  which  were  separately  examined. 
Examination  of  the  Steam  Distillate. 
The  distillate  was  a  milky  liquid,  on  the  surface  of  which  a  small 
quantity  of  a  greenish-yellow  essential  oil  had  separated.  It  was 
extracted  several  times  with  ether,  the  ethereal  liquid  washed  with 
water,  dried  with  anhydrous  sodium  sulphate,  and  the  ether 
removed.  The  residual  oil  was  distilled  under  the  ordinary  pressure, 
when  it  nearly  all  passed  over  between  2200  and  2300  C,  and 
amounted  to  19  grammes,  corresponding  to  0  25  per  cent,  of  the 
weight  of  dried  herb. 
This  essential  oil  had  a  brownish-yellow  color,  an  odor  resembling 
that  of  the  herb,  and  was  distinctly  camphoraceous.  It  was  readily 
soluble  in  70  per  cent,  alcohol,  and  its  solution  gave  a  slight  brown 
color  with  ferric  chloride.  It  possessed  the  following  constants  : 
d  1 5 °/  1 5 0  C.  ==  0-9500;  aD  -f  7°36/  in  a  I  dcm.  tube. 
The  aqueous  portion  of  the  distillate,  from  which  the  essential  oil 
had  been  removed  by  means  of  ether,  contained  a  small  amount  of  a 
mixture  of  acids,  which  was  found  to  consist  chiefly  of  formic  and 
butyric  acids. 
