260  Some  Constituents  of  Jambul.      {  Al?-J° 
J  1  Tune, 
ur.  Pharm. 
1917. 
The  proximate  analyses  were  conducted  in  accordance  with  the 
usual  methods,  and  gave  the  result  tabulated  below : 
Percent.  Percent 
Moisture    8.0  Protein    6.3 
Starch  (diastase)    41.4,  40.3     Ash    2.9 
Crude  fiber    2.3  Dextrin    2.1 
Pentosans   2.1  Tannin10    6.0 
The  quantitative  examination  of  the  alcohol-soluble  carbohy- 
drates resulted  as  follows : 
100  g.  of  Jambul  seeds  were  extracted  with  boiling  95  per  cent,  alcohol. 
The  alcoholic  extract  was  concentrated  to  a  syrup,  precipitated  with  a  slight 
excess  of  lead  subacetate  and  made  to  a  volume  of  200  Cc.  The  direct  and 
invert  readings  at  220  in  2  dcm.  tube  are  — 2.6V,  and  3.2V,  respectively..  The 
invert  reading  at  86°  in  a  2  dcm.  tube  was  0.35V.  Hence  sucrose  =  0.23  per 
cent.,  fructose  =  2.3  per  cent.,  and  glucose  ==  2.1  per  cent.,  respectively.  Gravi- 
metric determinations  by  the  Walker-Munson  process  gave  sucrose  0.33  per 
cent,  and  reducing  sugar  3.3  per  cent. 
(B)  Examination  of  Alcoholic  Extract. — For  this  purpose 
45.4  kg.  were  exhausted  by  percolation  with  wood  alcohol  at  room 
temperatures.  Power  and  Callan  extracted  the  seed  with  hot  ethyl 
alcohol.  The  percolate  (397  1.)  was  concentrated  under  diminished 
pressure  to  a  volume  of  12.5  liters.  This  concentrated  extract  on 
standing  deposited  230  g.  of  yellowish  material  which  was  quite  in- 
soluble in  the  usual  organic  solvents.  It  could  be  redissolved  in 
dilute  alkali  and  then  reprecipitated  by  the  addition  of  acetic  acid. 
After  being  digested  with  ether,  and  with  ethyl  acetate,  this  ma- 
terial was  crystallized  from  pyridine.  Brown  needles  were  ob- 
tained that  gave  the  characteristic  tests  for  ellagic  acid. 
The  filtered  alcohol  extract  was  poured  into  25  1.  of  distilled 
water  and  vigorously  agitated.  After  long  standing  the  resin  was 
removed  'by  filtration.  The  aqueous  alcohol  nitrate  was  concen- 
trated under  reduced  pressure  in  order  to  remove  the  alcohol. 
When  this  solution  was  diluted  with  distilled  water,  further  precipi- 
tation took  place  even  after  diluting  to  a  volume  of  80  liters.  The 
solution  was  allowed  to  stand  overnight  and  the  precipitate  (372  g.) 
was  filtered  off.  This  material  was  of  the  nature  of  a  phlobaphene. 
The  filtrate  was  concentrated  to  a  volume  of  9.77  1.  It  now  depos- 
ited 84  g.  of  ellagic  acid.    This  deposit  was  digested  with  ether  and 
10  Both  the  Hide  powder  method,  and  the  Proctor-Lowenthal  method 
gave  the  same  results. 
