262  Some  Constituents  of  Jambul.      {  AjuJ°u^arm- 
were  found.  Eighty-four  grams  were  hydrolized  by  boiling  for  one 
minute  with  10  per  cent,  potassium  hydroxide  solution.  The  mix- 
ture was  cooled  and  poured  into  an  excess  of  dilute  sulfuric  acid, 
and  then  steam  distilled.  From  the  contents  of  the  flask  a  quan- 
tity of  gallic  acid,  melting  at  240-242 °,  was  isolated. 
A  quantity  (171  g.)  was  boiled  with  a  large  volume  of  water 
and  then  vigorously  steam  distilled.  Ellagic  acid  separated.  The 
solution  was  concentrated  and  further  quantities  of  ellagic  acid 
separated.  At  length,  after  evaporation  to  dryness,  the  residue 
was  boiled  with  ethyl  acetate  and  some  insoluble  material  (ellagic 
acid)  was  removed  by  nitration.  It  was  impossible  to  obtain  crys- 
tals from  this  solution.  The  ethyl  acetate  solution  was  evaporated 
to  dryness,  and  again  taken  up  in  dry  ethyl  acetate,  in  which  it 
was  freely  soluble,  but  nothing  definite  could  be  obtained  from  it. 
The  amyl  alcoholic  extract  is  not  glucosidic. 
The  aqueous  liquid  which  had  been  extracted  with  ether,  chloro- 
form, and  with  amyl  alcohol,  was  freed  from  the  latter  immiscible 
solvent  by  a  vigorous  steam  distillation.  The  distribution  of  nitro- 
gen in  this  solution  was  as  follows :  Total  soluble  nitrogen,  0.0649 
per  cent. ;  ammonia  nitrogen,  0.0079  Per  cent. ;  lead  subacetate  pre- 
cipitable  nitrogen,  0.0197  per  cent. 
In  order  to  test  for  acid  amides,  one  fifth  of  the  solution  was 
precipitated  with  mercuric  acetate  solution,  but  the  results  were 
negative. 
The  remainder  of  the  solution  was  precipitated  with  basic  lead 
acetate,  filtered,  and  the  precipitate  was  found  to  consist  essentially 
of  lead  tannate. 
The  filtrate  from  the  lead  tannate  was  freed  from  lead  with 
hydrogen  sulfide  and  sharply  concentrated.  Although  this  syrup 
yields  a  precipitate  with  phosphotungstic  acid,  no  nitrogenous  bases 
were  isolated  from  this  fraction.  The  only  product  found  was 
sugar,  a  crystalline  deposit  of  a  J-phenylglucosazone  melting  at 
207-2080  being  readily  prepared.    Pentose  sugars  were  absent. 
The  Examination  of  the  Resin. — The  resin  which  precipi- 
tated when  the  alcoholic  extract  was  poured  into  water  weighed 
about  699  g.,  equivalent  to  1.5  per  cent,  of  the  drug.  It  was  dis- 
solved in  wood  alcohol,  poured  upon  purified  sawdust,  transferred 
to  a  continuous  extractor,  and  extracted  with  the  following  results : 
