266  Some  Constituents  of  Jambul.      {  Al?-  J°ur- pharm- 
J  June,  1917. 
factory  crystallization  could  be  obtained  even  from  very  concen- 
trated solutions.  The  alcoholic  solution  was  fractionally  precipi- 
tated with  an  alcoholic  solution  of  barium  acetate.  This  yielded 
Fractions  I  and  II.  Fraction  III  was  precipitated  by  the  addition 
of  water. 
1.  Melting  at  51-53°.    C,  75-8;  H,  12.4;  N.  v.,  204.3. 
III.  This  fraction  was  an  oil  and  gave  entirely  anomalous  ana- 
lytical data.  Iodine  no.,  35.2,  34.7;  neutralization  value,  34.9;  and 
saponification  value,  140.2. 
The  solid  acids  are  therefore  a  mixture  of  palmitic  and  stearic 
acids. 
Calc.  for  C16H3o02:  C,  75.0;  H,  12.5;  N.  v.,  219.1.  ClsH30O2:  C,  76.1;  H, 
12.7;  N.  v,  197.5. 
The  Ether  Extract  of  the  Resin,  which  amounted  to  20  g., 
contained  a  quantity  (2  g.)  of  an  insoluble  white  solid.  This  was 
filtered  off.  When  this  substance  was  dissolved  in  chloroform,  in 
the  presence  of  a  few  drops  of  acetic  anhydride,  and  sulfuric  acid 
was  added,  a  play  of  colors  resulted  showing  at  first  transient  pink, 
then  blue,  and  finally  a  beautiful  green.  It  was  crystallized  several 
times  from  dilute  pyridine,  and  then  melted  at  275-285  °.  It  was  a 
phytosterolin.  After  being  dried  to  constant  weight  at  1200  it  was 
analyzed. 
Calc.  for  C33H5G06:  C,  72.3;  H,  10.2.    Found:  C,  72.3;  H,  10.2. 
A  portion  of  this  was  converted  into  an  acetate,  which  crystal- 
lized from  dilute  alcohol  in  colorless,  glistening  leaflets  melting  at 
167-1680. 
0.5036  g.  of  the  anhydrous  phytosterolin  acetate,  when  made  up 
to  20  Cc.  with  chloroform,  showed  a  rotation  of  — 1.210  in  a  2 
dcm.  tube,  whence  [a]^=  —  24.... 
One  gram  of  this  phytosterolin  was  hydrolyzed  according  to  the 
method  outlined  by  Power  and  Salway.11  It  was  dissolved  in  60 
Cc.  of  hot  amyl  alcohol  and  20  Cc.  of  an  aqueous  15  per  cent,  solu- 
tion of  hydrochloric  acid  added,  together  with  sufficient  ethyl  alco- 
hol to  form  a  homogeneous  liquid.  After  heating  for  three  hours 
in  a  reflux  apparatus,  steam  was  passed  through  the  mixture  to  re- 
11 /.  Chem.  Soc,  103,  399  (1913)- 
