Am.  Jour.  Pharm.  \ 
March,  1894.  / 
Euparin. 
121 
Hot  concentrated  sulphuric  acid  gave  a  black  solution  of  the  sub- 
stance. Cold  acid  of  the  same  strength  gave  a  red  colored  solution, 
which  appeared  dark  yellow  in  thin  layers,  and  from  which  water 
precipitated  a  green  plastic  mass.  The  unfiltered  mixture  was 
shaken  with  ether  in  which  the  green  mass  was  insoluble,  and  with 
which  it  was  separated.  The  ether  was  allowed  to  evaporate  spon- 
taneously, and  the  residue  and  the  plastic  mass  were  treated  with 
boiling  alcohol,  which  dissolved  nearly  all  the  substance,  and  from 
which  it  was  deposited  upon  cooling. 
The  green  plastic  mass  and  the  residue  left  upon  the  evaporation 
of  the  ether  were  examined  for  sulphur  by  fusing  with  potassium 
hydrate  and  potassium  nitrate,  but  after  dissolving  the  products  of 
the  fusions  in  water  and  supersaturating  the  alkali  with  nitric  acid, 
no  precipitate  was  obtained  in  either  case  with  barium  chloride, 
indicating  the  absence  of  a  sulphur  derivative.  After  having  been 
boiled  with  one  per  cent,  sulphuric  acid  for  six  hours,  euparin 
retained  its  original  appearance  and  answered  its  usual  reactions. 
The  acid  liquid,  when  filtered  and  made  alkaline  with  sodium  hydrate 
gave  no  indication  of  glucose  when  heated  with  Fehling's  solution 
In  order  to  gain  some  idea  of  the  constitution  of  euparin,  the  fol- 
lowing substitution  products  were  made. 
CHLORINE  SUBSTITUTION  PRODUCT. 
Purified  chlorine  was  passed  into  a  solution  of  7  grams  of  the 
crystals  in  absolute  ether. 
Upon  standing,  the  liquid  deposited  nothing,  but  upon  sponta- 
neous evaporation,  a  thick  yellow  liquid  of  unstable  character  was 
obtained. 
This  became  hard  and  somewhat  brittle,  but  showed  no  signs  of 
crystallizing,  when  exposed  to  a  temperature  of —  16-6°  C. 
It  was  soluble  in  alcohol  from  which  it  separated  when  the  solu- 
tion was  chilled  to  —  150  C.  This  solution  gave  a  blood  red  color 
with  ferric  chloride. 
The  substitution  product  was  somewhat  darkened  by  treatment 
with  potassium  hydrate,  and  imparted  a  yellow  color  to  that  liquid, 
but  upon  acidification  with  nitric  acid  silver  nitrate  revealed  but 
traces  of  chlorine. 
Ammonium  hydrate  acquired  a  dark  red  color  when  brought  into 
contact  with  the  compound  which  it  partly  dissolved.    When  this 
