122 
Euparin. 
A.m.  Jour.  Pharm. 
March,  1894. 
liquid  was  made  acid  with  nitric  acid  and  silver  nitrate  was  added,  a 
bulky  precipitate  of  silver  chloride  was  produced. 
That  part  of  the  product  which  was  insoluble  in  ammonium 
hydrate,  had  a  grayish-black  appearance.  Estimation  of  the  chlor- 
ine in  the  substitution  product.  A  mixture  of  this  substance  and 
chlorine — free  calcium  carbonate  was  heated  to  redness  for  two  hours 
The  tube  and  its  cooled  contents  were  treated  with  water  and  then 
with  nitric  acid  in  excess.  The  solution  was  filtered  and  the  broken 
glass  rinsed  with  water  which  was  passed  through  the  filter  and 
added  to  the  solution  which  was  next  completely  precipitated  with 
silver  nitrate.  The  silver  chloride  was  collected,  washed  thoroughly 
and  dried  at  1300  C. 
4  142  grams  of  the  compound  gave  6  443  grams  of  silver  chloride. 
This  amount  of  silver  chloride  contains  1-5905  grams  of  chlorine  or 
38-48  per  cent,  of  the  weight  of  the  substitution  product. 
Assuming  that  chlorine  replaces  an  equal  number  of  hydrogen 
atoms  in  the  molecule  C12Hn03,  this  would  at  least  require  the  mole- 
cule C24H2206  in  which  seven  hydrogen  atoms  have  been  replaced, 
giving  C24H15C1706.  This  formula  represents  38  31  per  cent,  of 
chlorine. 
ACETYL  DERIVATIVE. 
Five  grams  of  euparin  were  boiled  with  5  grams  of  anhydrous 
sodium  acetate  and  10  cc.  of  acetic  anhydride  for  two  hours. 
When  the  resulting  liquid  was  poured  into  water  a  heavy  oily  layer 
separated.  This  was  washed  with  several  successive  portions  of 
water  and  finally  dried,  over  sulphuric  acid,  in  a  desiccator. 
When  cooled  to  —  150  C,  the  substance  became  a  crystalline 
mass,  but  reassumed  its  original  form  when  the  refrigeration  was 
discontinued,  and,  when  heated  in  a  paraffin  bath  to  1250  C,  it  was 
decomposed. 
When  heated  on  platinum  foil,  the  liquid  gave  off  an  acetous 
odor,  burned  with  a  luminous  flame  and  left  a  minute  residue  of 
odium  carbonate. 
The  sodium  salts  were  separated  by  treating  the  material  with 
later  and  shaking  the  mixture  with  other,  which  dissolved  the 
acetyl  compounds  and  upon  spontaneous  evaporation  left  it  in  the 
fluid  condition.  When  freed  from  sodium  salts,  the  product 
became  denser,  but  did  not  crystallize  at  a  low  temperature. 
