A¥SS.rm1  Euparin.  123 
A  second  quantity  of  the  substitution  product  was  made  in  the 
same  manner  as  employed  for  the  first,  but  with  the  omission  of 
the  sodium  acetate.  The  resulting  compound  had  the  general 
characters  of  the  previous  sample  and  became  dark  red — through 
the  composition — when  dried  over  calcium  chloride. 
On  account  of  the  unstable  character  of  this  substance,  no  definite 
knowledge  of  its  composition  was  gained  from  the  results  of  com- 
bustions and  of  estimations  of  acetyl  which  were  made. 
NITRO  COMPOUND. 
Euparin  was  treated  with  hot  nitric  acid  (sp.  gr.  1-40),  the  result- 
ing dark  red  liquid  diluted  with  water  and  the  copious  flocculent 
precipitate  obtained  was  dissolved  in  potassium  hydrate.  The 
alkaline  liquid  was  acidified  with  dilute  sulphuric  acid  and  shaken 
with  the  other,  to  which  it  imparted  a  yellow  color.  Upon  draw- 
ing off  and  evaporating  the  ether,  yellow  crystals  mixed  with  some 
brownish  substances  were  obtained. 
These  crystals  fused  and  became  black  between  1210  and  1230  C. 
They  were  soluble  in  water  and  the  yellow  color  of  the  solution 
was  intensified  by  alkalies. 
The  aqueous  solution  had  an  acid  reaction  and  a  bitter  taste.  It 
colored  the  skin  yellow  and  dyed  wool  and  silk  the  same  color. 
The  following  tests  for  picric  acid  were  obtained  with  the  water 
solution  : 
Normal  lead  acetate,  no  precipitate. 
Basic  lead  acetate,  yellowish  precipitate. 
Ammoniacal  copper  sulphate,  dark  green  color,  upon  standing  a 
precipitate  was  produced. 
Ferric  chloride,  brownish-red  precipitate. 
Potassium  cyanide,  purplish-red  color. 
Quinine  sulphate,  yellow  precipitate. 
ACTION  OF  FUSED  ALKALI. 
Euparin  was  gradually  added,  with  constant  stirring,  to  potassium 
hydrate  in  the  fused  state.  The  liquid  became  brownish-red  in  color 
and  emitted  an  apple  like  odor.  The  cold  mass  was  dissolved  in 
water,  the  solution  acidified  with  dilute  sulphuric  acid  and  shaken 
with  ether  which  removed  the  color,  and  upon  separation  and 
evaporation  left  a  dark  red-brown  residue.    This  was  dissolved  in 
