An  Ap°riir;i894arm' }  Analysis  of  Pycnanthemum  Linifolium.  171 
With  ferric  chloride  solution,  a  dark-green  coloration,  a  dark 
precipitate  afterwards  forming.  With  ferric  chloride  and  ferrous 
sulphate  solutions,  mixed,  the  same  coloration  as  with  ferric 
chloride  alone. 
With  gelatin  solution,  very  little  or  no  precipitate.  With  lead 
acetate  solution,  a  greenish-yellow  precipitate. 
Portions  heated  with  Fehling's  solution,  and  with  silver  nitrate 
solution,  caused  reduction.  These  reactions  agree  very  closely  with 
those  obtained  by  Dr.  Mohr  with  a  substance  extracted  by  him, 
which  he  supposed  to  be  a  tannin,  but  which  differed  from  the 
tannins  in  giving  no  precipitate  with  gelatin.  The  aqueous  and 
alkaline  aqueous  extracts  were  both  reddish  brown.  The  ash  of 
the  aqueous  extract  contained  a  considerable  amount  of  phosphoric 
acid. 
In  the  acid  aqueous  extract,  calcium  oxalate  was  estimated  by 
precipitating  one-tenth  by  -neutralizing  with  ammonia-water  and 
adding  alcohol ;  this  precipitate  was  incinerated,  and  the  ash  being 
assumed  to  consist  entirely  of  calcium  carbonate,  it  was  calculated  to 
calcium  oxalate.  The  amount  of  calcium  oxalate  thus  found  was 
equivalent  to  1-52  per  cent,  of  the  air-dry  herb. 
After  the  extraction  with  acid  water,  the  residue  of  the  plant  was 
boiled  with  water.  One-half  of  this  boiling  water  extract  was  acidi- 
fied with  about  one  per  cent,  of  hydrochloric  acid,  boiled  for  about 
four  hours,  made  alkaline,  and  the  reducing  power  estimated  with 
Fehling's  solution.  The  result  was  calculated  to  starch  or  inulin, 
and  as  a  test  for  starch  with  iodine  solution  in  a  portion  of  the  origi- 
nal extract  resulted  affirmatively,  the  quantity  is  stated  as  starch. 
The  presence  of  starch,  however,  of  course,  does  not  prove  the 
absence  of  inulin.  A  number  of  special  tests  for  tannin  were  made, 
using  portions  of  the  original  powdered  herb,  as  follows : 
A  portion  was  treated  with  cold  water,  a  brown  extract  being 
obtained,  which  gave  the  following  reactions  : 
WTith  ferric  chloride  solution,  mixed  solutions  of  ferric  chloride 
and  ferrous  sulphate,  and  with  ammonio-ferric  sulphate  solution, 
dark  green  precipitates. 
With  gelatin  solution,  no  precipitate. 
A  considerable  quantity  was  extracted  with  water,  and  the  filtered 
extract  divided  into  two  portions.  The  first  was  shaken  out  with 
acetic  ether,  the  separated  acetic  ether  solution  allowed  to  evapo- 
