172  Analysis  of  Pycnanthemum  Linifolium.  {AmA^\',isQtm' 
rate,  and  the  residue,  which  was  quite  small,  treated  with  a  mixture 
of  one  part  of  alcohol  with  about  three  of  ether,  which  left  almost 
nothing  undissolved.  The  residue  left  on  evaporation  of  this  ether- 
alcohol  solution  was  treated  with  water,  a  solution  being  produced 
with  which  the  following  reactions  were  obtained : 
With  a  mixture  of  ferric  chloride  and  ferrous  sulphate  solutions, 
a  dark-green  coloration,  the  fluid  afterwards  becoming  turbid. 
With  gelatin  solution,  no  precipitate. 
With  lead  acetate  solution,  a  yellow  or  greenish-yellow  precipitate. 
The  second  portion  was  treated  in  the  same  way  as  the  first,  except 
that  previous  to  the  agitation  with  acetic  ether  a  quantity  of  com- 
mon salt  was  added  to  the  fluid,  a  brown  precipitate  being  thereby 
produced.  The  same  tests  were  finally  applied  as  in  the  first  portion, 
with  the  same  results. 
Special  tests  were  also  made  with  a  portion  of  the  original  pow- 
dered herb,  for  alkaloids  and  glucosid£s,  which  were  not  detected. 
ADDITIONAL  NOTES  ON  P.  LANCEOLATUM. 
An  aqueous  extract  of  P.  lanceolatum  was  shaken  out  with  acetic 
ether,  etc.,  as  described  above  in  the  test  for  tannin  in  P.  linifolium. 
The  reactions  obtained  were  as  follows  : 
With  mixed  ferric  chloride  and  ferrous  sulphate  solutions,  a  dark- 
green  coloration. 
With  gelatin  solution,  no  precipitate. 
With  lead  acetate  solution  a  yellow  or  greenish-yellow  precipitate. 
The  same  substance,  therefore,  probably  occurs  in  both  species. 
The  detection  of  starch  in  P.  linifolium  led  to  the  belief  that  it 
could  probably  be  found  in  P.  lanceolatum,  and  a  number  of  tests, 
both  chemical  and  micro-chemical,  were  made. 
The  chemical  tests  were  made  in  extracts  prepared  by  boiling  the 
material,  previously  extracted  either  with  cold  water  or  with  dilute 
(about  O-i  per  cent.)  sodium  hydrate  solution,  followed  with  water, 
filtering  and  cooling;  but  gave  no  decisive  results;  the  various 
extracts  when  tested  with  iodine  solution  gave  colorations  which 
were  in  no  case  at  all  blue.  The  micro-chemical  tests  were  applied 
to  unprepared  material  and  to  samples  reserved  from  material  pre- 
pared as  above  stated,  by  extraction  with  water  and  dilute  soda 
solution.  Bodies  were  observed,  which  were  almost  certainly  starch 
granules,  but  were  quite  small  and  few  in  number. 
