Am■AJp0rilr;l894!rm•}      Analysis  of  Sanguinaria  Seed.  191 
The  acid  solution  rendered  alkaline,  the  alkaloids  removed  by 
shaking  with  a  mixture  of  ether  and  chloroform,  and  evaporated  in 
a  tared  beaker,  yielded  -025  gms.  of  alkaloid  in  the  form  of  a 
varnish,  equivalent  to  one-tenth  per  cent. 
The  mass  was  thoroughly  mixed  with  water,  and  acetic  acid  added 
until  a  solution  was  effected. 
This  solution  was  subjected  to  the  following  tests  which  are  very 
characteristic  of  sanguinarine.  The  solution  was  of  a  bright-red  color. 
With  tartar  emetic,  silver  nitrate,  mercurous  nitrate,  mercuric 
chloride  and  ferric  chloride,  it  yielded  a  yellow  precipitate  in  each 
case.  Gold  chloride  gave  a  dark  red-brown  precipitate,  potassium 
chromate,  a  yellow  and  iodine  a  crimson  precipitate. 
No  reaction  with  tannic  acid  or  basic  acetate  of  lead. 
The  original  drug  after  treating  with  ether  was  macerated  with 
absolute  alcohol,  yielding  1-468  gms.  of  extract. 
This  was  treated  with  fifty  cubic  centimeters  of  warm  water, 
filtered,  the  precipitate  taken  up  with  a  weak  solution  of  hydrochloric 
acid,  gave  the  red  color  of  sanguinarine  salts  and  responded  to  tests 
for  alkaloids.  A  small  portion  of  the  filtrate  from  the  above  gave  no 
reactions  for  tannic  or  gallic  acids. 
Another  portion  made  acid,  did  not  produce  the  red  color,  nor 
react  with  Mayer's  reagent  or  gold  chloride. 
The  alkaloidal  reactions  from  this  extract  were  very  faint, 
although  the  alkaloid  is  very  soluble  in  absolute  alcohol.  Their 
absence  at  this  point  was  due  to  the  fixed  oil,  extracted  by  petroleum 
ether,  being  a  perfect  solvent  for  them. 
The  residue  was  next  dried  and  extracted  with  distilled  water, 
yielding  1-86  gms.  of  extract,  consisting  of -3996  gms.  glucose,  4 147 
gms.  sugar,  -345  gms.  mucilage,  -360  gms.  dextrine  and  undeter- 
mined. 
The  sodium  hydrate  treatment  yielded  4-987  gms.  of  extract,  of 
which  2-856  gms.  were  pectin  and  albumen. 
From  the  hydrochloric  acid  macerations,  2-68  gms.  of  extract  were 
obtained,  1-45  gms.  of  which  was  calcium  oxalate  and  pararabin. 
The  drug  was  next  boiled  with  distilled  water,  yielding  an  extract 
which  gave  a  red-brown  reaction  with  solution  of  iodine,  showing 
the  absence  of  starch.  The  amount  of  extract  obtained  was  -830 
gms. 
The  remaining  portion  of  the  drug,  after  having  been  washed, 
