A&i^bSwLm}    On  Some  of  the  Tests  for  Quinine.  565 
(d)  10  cc.  of  chlorine  reagent:  no  coloration,  but,  after 
a  time,  the  mixture  assumes  a  yellow  hue. 
2.  1  cc.  of  a  i-5000th  solution,  with  one  drop  of  ammonia 
after  adding : 
(a)  One  drop  chlorine  water :  a  fine,  green  coloration, 
changing  to  bluish-green. 
(b)  01  cc.  chlorine  water:  a  pale  green  coloration. 
(c)  0  5  cc  chlorine  reagent :  a  light  green  color,  quickly 
changing  to  a  faintly  yellow. 
3.  01  cc.  of  I -5000th  solution,  with  one  drop  ammonia,  after 
adding : 
(a)  One  drop  chlorine  water  :  a  well-marked  green  color- 
ation. 
(b)  Two  drops  chlorine  water :  only  a  faintly  yellowish 
color. 
D.    i-iooooth  solution  of  quinine. 
1.  5  cc  of  the  solution  (=  0-5  mg.  quinine)  yields  with  one  drop 
of  ammonia,  after  addition  of : 
(a)  One  drop  chlorine  water :  the  mixture  slowly 
acquires  a  greenish  hue. 
(b)  Two  drops  chlorine  water  :  a  strongly  marked  green 
coloration. 
(c)  0-2  cc  of  chlorine  water  :  a  colorless  mixture. 
2.  1  cc  of  a  i-io,oooth  solution,  yields  with: 
(a)  One  drop  chlorine  water  and  one  drop  ammonia  : 
a  marked  green  coloration. 
(b)  Two  drops  chlorine  water  and  a  drop  of  ammonia  ; 
the  mixture  may  present  a  greenish  hue,  which  quickly 
changes  to  yellow. 
From  o-i  cc  of  a  i-iooooth  quinine  solution,  a  greenish  colora- 
tion may  be  obtained  by  employing  a  drop  of  a  much-diluted  solu- 
tion of  chlorine. 
From  5  cc  of  a  i-20000th  solution  of  quinine,  no  green  colora- 
tion was  obtained,  even  on  employing  a  diluted  solution  of 
chlorine. 
In  1872,  Prof.  Fliickiger1  proposed  to  substitute  bromine  for 
chlorine  in  this  test,  and  stated  that  its  reaction  was  much  more 
aNeues  Jahr.  f.  Pharm.,  1872.  139. 
