Am.  Jour.  Pharm.\ 
December,  1894.  / 
Rubiis  Villosus. 
581 
freshly  precipitated  ferric  hydrate,  until  no  reaction  for  tannin 
could  be  obtained  in  the  filtered  liquid.  The  detannated  filtrate  so 
obtained  was  concentrated  under  diminished  pressure  and  divided 
into  two  portions. 
One  of  these  evaporated  under  the  above  conditions  to  dryness, 
yielded  a  residue  of  a  reddish-brown  color. 
This  was  treated,  first  with  boiling  alcohol,  sp.  gr.  794,  which 
extracted  a  brownish-red,  bitter  substance,  and  then  with  ether, 
SP-  gr-  "725>  which  upon  evaporation  left  a  yellow,  inodorous,  acrid 
and  amorphous  body.  The  last-named  product  gave  at  once  with 
sulphuric  acid  a  deep-brown  color,  which  the  addition  of  water 
changed  to  greenish  and  finally  to  black.  The  same  effect  was 
produced  by  potassium  bichromate  and  sulphuric  acid.  No  change 
was  caused  by  nitric  acid  nor  by  ferric  chloride. 
The  brownish-red,  bitter  substance  extracted  by  the  boiling  alco- 
hol above,  was  dissolved  in  acidulated  water  and  the  solution  so 
obtained  repeatedly  shaken  with  chloroform,  which  removed  a  white 
substance,  the  following  reactions  of  which  gave  evidence  of  the 
presence  of  saponin. 
With  hot  sulphuric  acid  a  reddish-brown  color  was  produced. 
A  solution  of  the  material  in  water,  which  readily  dissolved  it, 
possessed  a  marked  tendency  to  froth,  and  gave  an  abundant  white 
precipitate  with  basic  lead  acetate  and  with  saturated  solution  of 
barium  hydrate. 
A  solution  of  the  principle  in  two  per  cent,  hydrochloric  acid 
when  boiled  for  several  hours  yielded  a  blackish  precipitate  and  a 
reddish-brown  liquid,  which  was  filtered  and  agitated  with  ether  and 
chloroform  in  succession. 
The  residues  left  upon  the  evaporation  of  these  solvents,  failed  to 
comply  with  the  tests  for  saponin  given  above,  thus  indicating  the 
decomposition  of  the  original  substance. 
The  remaining  portion  of  the  detannated  and  concentrated  liquid 
was  shaken  with  ether,  sp.  gr.  725.  This  solvent  left  upon  evapo- 
ration a  yellowish-white  residue,  which,  as  shown  by  the  following 
reactions,  corresponded  in  all  respects  to  the  villosin  of  Krauss. 
Heated  on  platinum  foil,  it  melted,  burned  with  a  yellowish  flame 
and  was  completely  consumed. 
It  was  soluble  in  methyl  and  ethyl  alcohol,  sparingly  in  water 
and  ether,  sp.  gr.  -725,  and  insoluble  in  chloroform. 
