146  Sterilisation  of  Adrenalin  Solutions.  {AmXp?1?|r'i9ih4arm' 
First  Series. 
In  the  first  experiment  12  ampoules  of  Adrenalin  Chloride  Solu- 
tion 1  in  10,000  were  used.  The  method  of  standardization  of  the 
solutions  which  was  used  in  this  as  well  as  in  all  the  following  ex- 
periments was  the  blood  pressure  method  3  in  which  the  solution  to 
be  tested  is  diluted  till  the  Adrenalin  Chloride  content  is  1  in  100,000. 
This  dilution  is  then  tested  in  comparison  with  a  1  in  100,000  solu- 
tion which  is  made  up  from  an  accurately  weighed  amount  of  C.  P. 
Adrenalin  crystals.  One  ampoule  in  this  series  was  used  as  a 
control  and  its  activity  found  to  be  equal  to  standard.  The  other 
eleven  were  placed  in  boiling  water,  one  ampoule  being  removed 
at  the  end  of  each  15  minutes  and  tested.  Each  was  found  to  be  of 
standard  activity. 
This  experiment  serves  to  show  that  the  activity  of  Adrenalin 
Chloride  Solution  in  ampoules  is  not  impaired  by  sterilizing  in  boil- 
ing water  for  any  period  of  time  up  to  3  hours. 
Second  Series. 
A  second  lot  of  ampoules  of  Adrenalin  Chloride  Solution  was 
used,  one  ampoule  as  before  being  reserved  as  a  control.  The 
others  were  sterilized  for  periods  of  15  minutes  each  and  one  was 
removed  and  tested  after  each  sterilization. 
The  ampoules  for  further  sterilization  were  cooled  to  room  tem- 
perature before  being  sterilized  again  and  the  process  was  not 
repeated  oftener  than  twice  a  day  so  that  considerable  time  elapsed 
between  two  sterilizations. 
Results  were  as  follows : 
Ampoule.  Sterilized.  Activity. 
No.  i  Not  sterilized  Standard 
No.  2  Once  (15  min.)  Standard 
No.  3  Twice  Standard 
No.  4  3    times  Standard 
No.  5  4    times  Standard 
No.  6  5    times  Standard 
No.  7  6    times  Standard 
No.  8  7    times  Standard 
No.  9  8    times  90  per  cent. 
No.  10  9   times  80  per  cent. 
No.  11  10  times  80  per  cent. 
No.  12  11  times  80  per  cent. 
