148 
Sterilisation  of  Adrenalin  Solutions. 
\  Am.  Jour.  Pharm. 
t      April,  1914. 
The  results  of  the  above  experiments  were  all  checked  and  are 
summarized  in  the  following  table : 
Solution  of  Adrenalin  Chloride. 
Solution  of  Synthetic  Sub- 
First  Method : 
Cotton  plugged  > 
tube  in  boiling  j 
water  15  minutes  j 
at  a  time.  J 
Second  Method  : 
Tightly  stop- 
pered  bottle 
in  boiling  water  [ 
15  minutes  at  a  time.  J 
Third  Method : 
Boiled  5 
minutes  over 
flame  in  open 
flask. 
1st  ster. 
per 
1.  IOO 
2.  IOO 
1.  IOO 
2.  IOO 
1.  IOO 
2.  IOO 
2nd. 
per 
IOO 
IOO 
IOO 
IOO 
IOO 
IOO 
3rd. 
per 
4th. 
per 
1st  ster. 
per 
stance. 
2nd. 
per 
cent.  cent. 
3rd.  4th. 
per  per 
cent.  cent. 
90 
90 
IOO 
90 
90 
IOO 
80 
90 
90 
90 
75 
90 
1.  100     100     80  80 
1.  IOO 
2.  IOO 
1.  IOO 
2.  IOO 
IOO 
90 
90 
90 
70 
80 
80 
80 
70 
75 
80 
The  results  obtained  show  that  Adrenalin  Chloride  Solution  1  in 
1000,  containing  no  preservative  other  than  a  slight  excess  of  hydro- 
chloric acid,  can  be  sterilized  at  least  twice  by  heating  to  the  tem- 
perature of  boiling  water  under  various  conditions  without  losing 
any  activity.  After  this  the  loss  is  quite  gradual,  so  that  it  prob- 
ably would  not  be  noticeable  therapeutically  until  after  the  fourth 
or  fifth  sterilization.  By  a  comparison  of  the  results  obtained -with 
Adrenalin  Chloride  Solution  and  the  solution  of  the  synthetic  sub- 
stance it  can  be  seen  that  the  loss  of  activity  due  to  sterilization 
occurs  more  quickly  in  the  case  of  the  latter  solution  and  also  that 
the  deterioration  is  more  marked. 
Summary. 
1.  Adrenalin  Chloride  Solution  in  ampoules  can  be  heated  con- 
tinuously for  3  hours  to  the  temperature  of  boiling  water  without 
any  loss  of  activity. 
2.  Adrenalin  Chloride  Solution  in  ampoules  can  be  sterilized  by 
immersion  in  boiling  water  for  seven  distinct  periods  of  15  minutes 
each  without  loss  of  activity. 
