Amjufy?i'9iP4harm'}  Estimation  of  Morphine  in  Pills,  Etc.  311 
the  alcohol  chloroform  mixture  and  then  with  three  10  c.c.  portions 
of  chloroform. 
Evaporate  the  alcohol  chloroform  solution  on  a  water-bath  under 
a  current  of  warm  air  to  dryness,  add  a  few  cubic  centimetres  of 
alcohol  and  again  evaporate.  Dissolve  the  residue  in  an  excess  of 
n/io  acid  and  titrate  back  with  N/50  alkali,  using  cochineal  as  in- 
dicator. Each  cubic  centimetre  of  acid  neutralized  by  the  alkaloid  = 
0.0301  Gm.  of  crystalline  morphine  or  0.0376  Gm.  morphine  sulphate. 
Following  are  some  of  the  results  obtained  by  this  method.  A 
2  per  cent,  solution  of  morphine  alkaloid  was  prepared  and  assayed. 
(  0.0976  Gm.  =  97.6    per  cent. 
"5  c.c.  =  0.1  Gm.  morphine  gave  {         ,  ^    ,  ^ 
°  .    fi  {  0.0976  Gm.  —  97.6    per  cent. 
  ,•  f  0.1940  Gm.  —  97.0    per  cent. 
10  c.c.  —  0.2  Gm.  morphine  gave  J      ^  * 
{  0.1952  Gm.  =  97.59  per  cent. 
20  c.c.  =  0.4  Gm.  morphine  gave  {  °-390i  Gm.  =  97.52  per  cent. 
10.3901  Gm.  =  97.52  per  cent. 
A  2  per  cent,  solution  of  commercial  morphine  sulphate. 
j  0.09944  Gm.  =  99.44  per  cent. 
5  c.c.  =  0.1  Gm.  morphine  sulphate  gave....!  ^   
J  .  "  (  0.09944  Gm.  —  99.44  per  cent. 
^  u-         1  u  j.  f  0.1981    Gm.  =  99.06  per  cent. 
10  c.c  =  0.2  Gm.  morphine  sulphate  gave....  \      y  ^  F 
{  0.1981    Gm.  =  99.06  per  cent. 
20  c.c.  =  0.4  Gm.  morphine  sulphate  gave...  j  °-3932    Gm.  =  98.3"  per  cent. 
I  0.3940    Gm.  =  98.5    per  cent. 
The  quantity  of  solvent  used  in  the  extraction  of  this  sample  was 
the  same  in  each  case. 
Two  weighed  quantities  of  another  sample  of  morphine  sulphate 
0.0535  and  0.0560  Gm.  gave  0.05395  and  0.05634  Gm.  respectively 
when  extracted. 
A  quantity  of  morphine  alkaloid  (1  Gm.)  was  dissolved  in  ex- 
actly 50  c.c.  n/io  acid,  then  made  up  to  100  c.c.  with  distilled  water ; 
of  this  solution  two  10  c.c.  portions  were  titrated  and  required  for 
neutralization  8.7  c.c.  and  8.7  c.c.  of  N/50  alkali,  showing  the  pres- 
ence of  0.09813  Gm.  of  morphine  in  each.  Two  other  10  c.c.  por- 
tions extracted  by  the  above  method,  the  residue  dissolved  in  5  c.c. 
n/io  acid  and  titrated,  required  8.7  c.c.  and  8.8  c.c.  of  N/50  alkali 
to  neutralize,  corresponding  to  0.09813  Gm.  and  0.09752  Gm.  re- 
spectively. 
