As™ptemberf i9iT' }    Insecticidal  Value  of  Larkspur  Seed.  415 
by  evaporation  on  the  water-bath  and  the  residue  dissolved  in  95  per 
cent,  alcohol. 
No.  5. — Extracted  with  petroleum  benzine,  the  benzine  solution 
shaken  out  with  dilute  acid  to  remove  the  greater  part  of  the  alkaloid, 
then  evaporated  and  the  residue  dissolved  in  95  per  cent,  alcohol. 
No.  6. — Extracted  with  10  per  cent  acetic  acid,  the  acid  removed 
by  distillation  and  the  residue  dissolved  in  dilute  alcohol. 
No.  7. — The  drug  residue  from  No.  6  extracted  with  95  per  cent 
alcohol. 
No.  8. — Extracted  with  95  per  cent,  alcohol  until  a  yield  of  1  c.c. 
for  each  gramme  of  drug  used  was  obtained.  This  gave  a  perfectly 
clear  fluid,  showing  no  signs  of  separating  after  standing  several 
weeks. 
No.  9. — The  extraction  of  drug  residue  of  No.  8  continued  until 
a  further  yield  of  1  c.c.  for  each  gramme  of  drug  used  was  obtained. 
No.  10. — The  alkaloidal  residues  of  several  assays  dissolved  in 
sufficient  95  per  cent,  alcohol  to  make  a  1  per  cent,  solution. 
These  fluidextracts  varied  in  color  from  a  dark  brown  (No.  6)  to 
a  very  light  yellow,  and  after  standing  several  weeks,  with  the  excep- 
tion of  Nos.  2,  3,  and  6,  which  show  some  sediment,  are  in  good 
condition. 
The  alkaloid  and  fixed  oil  contents  are  as  follows : 
Color  Alkaloid  Oil 
f  i— A  Pale  yellow  0.26  21.34 
\  i-B  Yellow  0.81  7.08 
2  Dark  brown          1.42  0.19 
3  Dark  brown          1.26  0.12 
4  Pale  yellow  0.17  30.37 
5  Pale  yellow  0.06  30.54 
6  Very  dark  brown  1.24  0.14 
7  Yellow  0.06  23.67 
8  Yellow  0.60  24.76 
9  Pale  yellow  0.11  3.60 
10  Reddish  yellow  1.0  (not  assayed) 
The  drug  itself  assayed  1.78  per  cent,  alkaloids  and  36.1  per  cent. 
oil. 
The  insecticidal  values  of  the  fluids  were  determined  by  Mr.  H. 
C.  Hamilton  by  the  method  of  Houghton  &  Hamilton.*  The  results 
were  as  follows : 
*  Eleventh  Report  of  the  Michigan  Academy  of  Science,  1909. 
