Am.  Jour  Pharra. \ 
October,  1900.  J 
Assay  of  Drugs. 
In  the  experiments  in  the  first  table  on  p.  480,  alcoholic  solutions 
of  brucine  sulphate  containing  ^  grain,  grain,  1  grain  and  \ 
grain  of  the  alkaloid  to  50  c.c.  of  the  solution  were  used. 
In  these  experiments  with  brucine,  alcohol  and  water,  we  again 
notice  an  increase  in  growth  over  the  experiments  with  strychnine, 
thus  tending  to  show  that  brucine  is  a  less  powerful  alkaloid  upon 
plants  than  strychnine. 
The  succeeding  table  gives  the  results  of  the  experiments  at  the 
end  of  the  second  twenty-four  hours. 
In  the  second  twenty-four  hours  the  same  conditions  remain 
throughout  the  experiment ;  the  maximum  growth  occurred  in  the 
solution  containing  -fc  grain  and  death  in  the  1  grain  solution. 
In  the  following  table  are  given  the  results  with  alcoholic  solu- 
tions of  strychnine  nitrate  and  brucine  sulphate,  containing  ^  grain, 
j1^  grain,  -J.  grain  and  -J-  grain  of  the  alkaloids  in  equal  proportion 
to  50  c.c.  of  the  solution  : 
STRYCHNINE  NITRATE),  BRUCINE)  SULPHATE)  AND  E)THYL,  At,COHOI,. 
Strychnine  nitrate  .  .  .0-15  gin. 
Brucine  sulphate  ....  0*15  gm. 
Alcohol,  94  per  cent.  .  .  75  c.c. 
Water  25  c.c. 
'  Temperature  (i6°-2i°  C).  First  24  Hours. 
Pisum  Sativum. 
IvUPINUS  ALBUS. 
Per  Cent. 
Actual 
Growth. 
Remarks. 
Actual 
Growth. 
Remarks. 
,V  gr.  in  50  c.c.  of 
sol.  or  1  c  c.  sol. 
HaO  q  s  50  c.c. 
f    6'  mm. 
1    7"  " 
j 
| Apparently  normal 
f  io*  mm. 
1  Apparently  .normal. 
XV  gr.  in  50  c.c.  of 
sol.  or  2  c.c.  sol. 
H20  q  s.  50  c.c. 
{  ;: : 
)  -  - 
1 5- :: 
l  5'5 
,  .. 
1  gr.  in  50  c.c.  of 
sol.  or  2^c.c.  sol. 
H20  q.s.  50  c.c. 
! : : 
1  - 
f    3"5  " 
I    3'5  " 
1 
1 
1  gr.  in  50  c.c.  of 
sol.  or  4  c.c.  sol. 
H20  q.s.  50  c.c. 
|no  growth 
1 
|       Dead,  flabby 
|  No  growth 
\      Dead,  flabby. 
J 
Comparing  the  above  experiments  with  those  of  strychnine  and 
alcohol  and  brucine  and  alcohol,  we  observe  an  increase  in  the 
growth  of  the  seedlings  over  that  in  strychnine  and  alcohol,  and  a 
slight  decrease  under  that  in  brucine  and  alcohol. 
