Am.  Jour.  Pharin.\ 
October,  1900.  J 
Assay  of  Drugs, 
483 
In  the  second  twenty-four  hours  the  same  conditions  are  evident. 
In  the  second  table  on  p.  482  are  given  the  detailed  results  with  a 
tincture  of  nux  vomica  of  U.S. P.  strength  (0-3  gramme  of  total  alka- 
loids in  100  c.c.  of  tincture)  from  which  the  oil  had  been  extracted. 
13*332  grammes  of  the  powdered  drug,  the  amount  used  in  making 
100  c.c.  of  the  U.S. P.  tincture,  contained  750  gramme  of  oil  or  5-62 
per  cent. 
In  the  foregoing,  it  is  seen  that  no  growth  of  the  radicle  occurred 
in  the  solution  containing  4  c.c.  of  the  tincture,  and  that  between 
4  c.c.  and  ^  c.c.  there  was  a  gradual  increase  in  length  according 
as  the  strength  of  tincture  in  the  solution  was  decreased. 
In  the  following  experiments  are  given  the  results  at  the  end  of 
the  second  twenty-four  hours  : 
TINCTURE  OF  NUX  VOMICA  (FREE  FROM  FAT). 
Temperature  (i6°-2i°  C).  Second  24  Hours. 
Pisum  Sativum. 
IyUPINUS  ALBUS. 
Per  Cent. 
Actual 
Growth. 
Remarks. 
Actual 
Growth. 
Remarks. 
5V  gr.  alkaloids  in 
50  c.c.  or  \  c.c. 
tinct.H20  q.s.50  c.c. 
|    5-  mm. 
^Apparently  normal 
(  15-  mm. 
1  15-5  " 
j-Apparently  normal. 
?V  gr.  alkaloids  in 
50  c.c.  or  \  c.c. 
tinct.H20  q.s.50  c.c. 
(    3"5  " 
t    3'5  " 
}  -  ■ 
{""■■ 
} 
3V  gr.  alkaloids  in 
50  c  c.  or  1  c.c. 
tinct.H20  q.s.50  c.c. 
C : 
}  -  - 
{ :  :- 
(.  :  ■ 
xl„  gr.  alkaloids  in 
50  c.c.  or  2  c.c. 
tinct.H20  q.s.50  c.c. 
{ ;; : 
j*No  further  growth 
{ 7  ■ 
j-  No  further  growth. 
|  gr.  alkaloids  in 
50  c.c.  or  2\  c.c. 
tinct.HaO  q.s.50  c.c. 
C : 
} 
i: : 
} : 
\  gr.  alkaloids  in 
50  c.c.  or  4  c.c. 
tinct.H20  q.s.50  c.c. 
-|no  growth 
|       Dead,  flabby 
-|no  growth 
j-      Dead,  flabby. 
The  seedlings  in  the  solutions  containing  y£  c.c,  j£  c.c.  and  I  c.c. 
of  the  tincture  show  a  slight  increase  in  growth  during  the  second 
twenty-four  hours,  while  those  in  solutions  containing  2  c.c.  and 
2y2  c.c.  show  no  further  growth. 
The  following  table  gives  the  results  with  a  tincture  of  nux 
vomica  U.S. P.  strength  (0-3  gramme  of  total  alkaloids  in  100  c.c. 
of  tincture)  from  which  the  fat  has  not  been  extracted  : 
