700  Determination  of  Morphine.  ^>m.jour.^Pharm. 
present  was  smaller,  in  this  than  in  the  preceding  experiment.  Mor- 
phine was  dissolved  in  2.0  Cc.  N  sulphuric  acid  and  precipitated  by 
8.0  Cc.  of  -f^N  ammonium  hydroxide  in  the  presence  of  2.5  Cc.  of 
alcohol  and  5.0  Cc.  of  ether.  The  remainder  of  the  manipulation  re- 
sembled that  of  Experiment  9. 
Titration  results  : 
Total  acid  used  =  15.00  Cc. 
Acid  equivalent  to  the  alkali  =  5.75  Cc. 
Acid  equivalent  to  the  morphine  =  9.25  Cc. 
Since  1  Cc.  of  this  standard  acid  =  0.0300  Gm.  morphine 
Weight  of  morphine  found  =  0.2775  Gm. 
Weight  of  morphine  originally  taken  =  0.3000  Gm. 
Loss  in  precipitation  =  0.0225  Gm. 
Experiment  11. — In  this  case  neither  alcohol  nor  ether  were 
present  during  the  precipitation,  their  place  being  taken  by  chlo- 
roform.   The  time  allowed,  for  precipitation  was  greatly  reduced.' 
Crystallized  morphine  (0.3000  Gm.)  was  dissolved  in  ^  sul- 
phuric acid  and  the  solution  was  titrated  with  standard  sodium 
hyroxide. 
Total  acid  used  =25.00  Cc. 
Acid  equivalent  to  the  alkali  =  15.13  Cc. 
Acid  equivalent  to  the  morphine  =  9.87  Cc. 
A  drop  of  hydrochloric  acid  was  added  to  the  titrated  solution, 
which  was  then  eA^aporated  to  dryness.  The  residue  was  dissolved 
in  5.0  Cc.  of  water,  to  which  was  then  added  5.0  Cc.  of  chloroform 
and  enough  strong  ammonium  hydroxide  solution  to  make  the  mix- 
ture alkaline.  Crystallization  of  morphine  was  promoted  by  stir- 
ring the  liquid  and  scratching  the  sides  of  the  containing  vessel  with 
a  rod.  It  was  allowed  to  stand  3I/2  hours  from  the  first  appearance 
of  a  precipitate.  It  was  then  filtered  through  a  cotton  plug  and  the 
morphine  washed  with  10-15  Cc.  of  water.  The  morphine  was  dis- 
solved in  dilute  acid.  The  acid  solution,  with  the  cotton  plug,  was 
transferred  to  a  separating  funnel,  made  alkaline,  and  extracted  as 
in  Experiment  5.  The  morphine  thus  separated  was  titrated  with 
■^q  sulphuric  acid  and  sodium  hydroxide. 
Total  acid  used 
Acid  equivalent  to  the  alkali 
Acid  equivalent  to  the  morphine 
=  26.50  Cc. 
=  16.89  Cc. 
=  9.61  Cc. 
