86o 
Determination  of  Morphine. 
Am.  Jour.  Pharm. 
Dec,  1918. 
morphine  1.14  per  cent.  This  was  placed  in  a  graduated  (200  Cc.) 
flask  and  digested  on  the  water  bath  with  20  Cc.  of  water  for  10 
minutes.  An  aqueous  solution  containing  9  Gm.  of  barium  chloride 
was  added,  followed  by  120  Cc.  of  a  cold  saturated  barium  hydroxide 
solution.  After  standing  30  minutes  the  mixture  was  diluted  to  200 
Cc.  The  remainder  of  the  determination  was  carried  out  in  the 
manner  already  described,  but  100  Cc.  was  substituted  for  50  Cc.  and 
no  Cc.  for  55  Cc.  when  taking  an  aliquot  part  and  later  diluting. 
Titration  Results. 
Total  acid  used  =  10.00  Cc. 
Acid  equivalent  to  the  alkali  =  9.27  Cc. 
Acid  equivalent  to  the  morphine  ==  0.73  Cc. 
Corresponds  to  0.0219  Gm.  of  crystalline  morphine,  or  0.80  Per  Cent. 
Calculated  from  Experiments  31,  32  and  33,  the  result  should 
have  been  1.14  per  cent.,  i.  e.,  0.34  per  cent,  higher. 
Titration  with  acid  of  anything  like  decinormal  strength  is  obvi- 
ously not  a  suitable  method  for  the  accurate  determination  of  such 
small  amounts  of  morphine  as  were  present  in  this  case  and  in  Ex- 
periment 37,  but  it  serves  sufficiently  to  show  how  far  the  present 
method  of  separating  morphine  can  be  applied. 
If  the  correction  mentioned  on  p.  856  be  applied  to  Experiments 
31,  32,  33,  37  and  38,  we  get  figures  in  closer  agreement,  as  follows. 
Experiment  31.      Morphine  11.67  Per  Cent.  ~) 
32.  11.60  Per  Cent.  j>    Mean       11.70  Per  Cent. 
33.  "         11.83  Per  Cent.  J 
"  37-  i-io  Per  Cent.  )    ~  ,    .   •  ,         _  ~ 
„  •  „    L   }    Calculated  1.17  Per  Cent. 
"  38.  1.09  Per  Cent,  j 
Acknowledgment. 
In  the  course  of  this  work,  which  was  commenced  in  China  in 
1909,  I  have  received  direct  and  indirect  help  from  friends  and 
colleagues  too  numerous  to  mention,  but  notably  from  Mr.  F.  W. 
Babington,  chief  analyst  in  this  laboratory,  Mr.  Allan  A.  Ferguson, 
at  one  time  a  member  of  its  staff,  and  Hu  Ch'ing  T'ang. 
Without  the  unfailing  kindness,  consideration,  and  helpfulness  of 
the  former,  the  work  could  not  have  been  continued.  Mr.  Fergu- 
son assisted  with  many  experiments  under  my  supervision:  none  of 
