582  Relative  Activity  of  Digitalis.  {Arr 
August,  1 918. 
Table  II. 
Guinea-pig  Assay  of  Petioles  Separated  from  Select  Cleaned  Digitalis — 
Ten  Per  Cent.  Infusion. 
Date  and  Time 
of  Test. 
No.  of 
.  Pig. 
Weight 
of  Pig. 
Dose  of 
Drug  in 
Gm.  per 
250  Gm. 
W t.  of  Pig. 
Dose  of 
Drug"  in 
Gm.  per 
Gm.  Wt. 
of  Pig. 
Actual 
Dose 
Given  in 
Mis.  of 
10  %  Inf. 
Results. 
2-4-18,  I  :o5  A.M.  . 
7 
575 
•4 
.OOl6 
9.2 
Died,  2-4-18,  1 :45 
a.m.,  40  min. 
2-4-18,  10:30  P.M. 
13 
335 
•3 
.0012 
4.02 
Died,  2-5-18,  12:30 
a.m.,  2  hrs. 
2-4-18,  10:35  P.M. 
14 
390 
•25 
.00010 
3-9 
Died,  2-5-18,  12  m., 
13  hrs.  25  min. 
3-27-18,  4:15  P.M. 
5° 
355 
.25 
.0010 
3-55 
Died,  3—28—18,  8:25 
a.m.,  16  hrs.  10  min. 
4-18— 18,  2:46  A.M. 
00 
205 
•2375 
•00C95 
1.94 
Died,  4— 19— 18,  12:00 
p.m.,  33  hrs. 
4-1 8-1 8,  2:44  A.M. 
* 
59 
260 
•2375 
.00C95 
2-47 
Died,  4— 18— 18,  9:00 
a.m.,  6  hrs.  16  min. 
4— 18— 18,  2:41  A.M. 
58* 
560 
.2250 
.ooc9t 
5-04 
-Uieu,  4—  io— io,  9-00 
a.m.,  6  hrs.  16  min. 
4— 18— 18,  2:38  A.M. 
57 
540 
.2250 
.ooc9f 
4.86 
Died,  4— 18— 18,  9:00 
a.m.,  6  hrs.  16  min. 
4-l8-l8,  2:35  A.M. 
56* 
310 
•  2125 
.00085 
2.63 
Recovered 
4-I8-I8,  2:30  A.M. 
55* 
320 
.2125 
.00085 
2.72 
Recovered 
4-18-18,  2:25  A.M. 
54* 
435 
.2 
.ooc8 
3-48 
Recovered 
2-4-18,  1:15  A.M.  . 
8 
380 
.2 
.0008 
3-04 
Recovered 
3-27-18,  4:05  P.M. 
45 
350 
.2 
.0008 
2.8 
Recovered 
3-27-18,  4:11  P.M. 
49* 
375 
.2 
.0008 
3.00 
Recovered 
3-28-18,  9:35  P.M. 
51* 
260 
.2 
.0008 
2.C8 
Recovered 
3-27-18,  4:07  P.M. 
48* 
440 
•175 
.0007 
3-o8 
Died,  3-27-18,  7:30 
p.m.,  3  hrs.  23  min. 
3-28-18,  9:40  P.M. 
52* 
475 
•175 
.0007 
3-325 
Recovered 
3-28-18,  9:45  P.M. 
53* 
180 
•175 
.0007 
1.26 
Recovered 
A  ten  per  cent,  infusion  of  the  number  50  sittings  which  ran 
56.81  per  cent,  of  ash  was  prepared  and  also  tested  by  the  guinea- 
pig  method.   The  results  were  as  follows: 
*  The  10  per  cent,  infusion  used  for  these  pigs  was  prepared  from  petioles 
rolled  between  the  fingers  in  order  to  free  them  from  about  5  per  cent,  of 
laminar  portions. 
t  M.  L.  D.  is  .225  Gm.  of  petiole  per  250  Gm.  wt.  of  guinea-pig,  or  .0009  of 
petiole  per  Gm.  wt.  of  pig. 
