AMin*,i?72RM'}    Lycopersicum  Esculentum — Tomato.  199 
The  filtrate  from  this  was  evaporated  and  boiled  until  the  calcium 
citrate  was  precipitated,  which  was  then  washed  with  hot  water,  on 
the  filter,  mixed  with  a  small  quantity  of  pure  water,  and  decomposed 
by  dilute  sulphuric  acid ;  the  calcium  sulphate  was  allowed  to  deposit, 
the  liquid  was  filtered,  concentrated  and  set  aside. 
After  three  days  a  number  of  crystals  were  found  deposited,  but 
mixed  with  some  viscid  coloring  matter. 
The  mother  liquor  was  decanted,  and  after  concentration  was  set 
aside  for  further  crystallization.  The  first  crystals  were  dissolved  in 
a  small  quantity  of  distilled  water,  the  solution  filtered,  concentrated 
and  set  aside.  After  standing  twenty-four  hours  no  crystals  had 
formed  in  either  liquid.  They  were  then  mixed,  concentrated  and 
filtered.  After  standing  about  two  weeks  a  small  quantity  of  crystals 
had  formed. 
These  were  separated  and  the  mother  liquor  again  concentrated, 
when  a  very  small  product  was  obtained. 
Owing  to  press  of  other  duties  I  did  not  purify  the  resulting  crys- 
tals. The  yield  was,  however,  very  slight,  being  probably  ten  grains 
from  one  gallon  of  fresh  juice,  equal  to  about  nine  pounds  of  fruit. 
To  obtain  the  malic  acid,  the  filtrate  left  after  precipitation  of  cal- 
cium citrate  by  boiling  was  concentrated,  and  calcium  malate  was 
separated  by  the  addition  of  alcohol.  Allowing  it  to  subside,  it  was 
separated  by  filtration  and  the  filtrate  again  treated  with  alcohol  to 
separate  any  remaining  malate.  A  small  quantity  was  obtained,  and 
after  filtration  it  was  mixed  with  the  first  product.  This  was  then 
dissolved  in  a  small  quantity  of  hot  water,  the  solution  filtered  and, 
after  partial  evaporation,  mixed  with  an  equal  bulk  of  alcohol,  and 
the  mixture  treated  with  dilute  sulphuric  acid. 
The  resulting  calcium  sulphate  was  separated  after  it  had  com- 
pletely subsided,  and  the  filtrate,  containing  malic  acid  in  dilute  alco- 
holic solution,  was  partially  concentrated.  After  standing  twenty- 
four  hours,  no  crystals  having  appeared,  it  was  further  concentrated 
and  set  aside. 
After  standing  about  two  weeks,  I  found  it  had  deposited  a  quan- 
tity of  colored  inert  matter.  It  was  again  partially  evaporated  and 
filtered,  and  in  a  few  days  a  quantity  of  crystals  were  obtained. 
While  engaged  in  purifying  these  with  animal  charcoal  the  entire 
product  was  lost  by  the  accidental  breaking  of  the  capsule  containing 
the  solution,  so  that  I  was  unable  to  ascertain  the  exact  weight  of  the 
purified  product.    It  was,  however,  larger  than  that  of  citric  acid. 
