ON RUNNET AND CHYMOSINE. 
195 
I evaporated to dryness, 30 grs. of runnet, over sulphuric 
acid, and obtained a very active runnet. 
Ten drops of runnet, put into 125 grs, of boiling milk pro- 
duced no action. 
I mixed 15 drops of runnet with 125 grs. of cold milk, and 
carried it to the boiling point as soon as possible; the milk 
was not altered. 
Some runnet was saturated with calcined magnesia, three 
drops coagulated 250 grs. of milk. 
I made the runnet alkaline with bi-carbonate of soda, and 
poured it into some milk; coagulation ensued. 
I placed some runnet in a matrass, and heated on a salt- 
water bath; it began to loose its clearness at 45° c, and the 
cloud increased up to 60° c. Three drops of runnet, at 45° c, 
and eight drops, at 50° c, each separately, coagulated 125 grs. 
of milk. The coagulation with the latter, was more slow 
than with the former ; the slowness of the operation is indi- 
cated by the amount of cream which separates. A coffee 
spoonful of the runnet, heated to 60° c, caused no change in 
125 grs. of milk. 
To ascertain which portion of the stomach produces the 
runnet, I dissected off the mucous membrane from a stomach, 
and placed the serous united to the muscular membrane, in 
300 grs. of alcohol of 47° c; after a maceration of three months 
I mixed, without any result, 20 grs. of this liquor with 125 
grs. of milk. The mucous membrane was placed in 130 grs. 
of alcohol, of 27° c. After a month's maceration, the liquid 
had but a feeble action, for it required 20 drops to coagulate 
a pound of milk. I placed in the flask a little chloride of 
sodium, and obtained an active runnet ; one drop coagulated 
170 grs. of milk. 
The mucous membrane of the stomach of a calf, which had 
been macerated in 375 grs. of alcohol of 27 c, and which 
produced a feeble effect; was withdrawn fiom the flask after 
macerating one month, was washed and placed in a flask with 
alcohol of 27° c. and 6 grs. of salt; the next day three drops 
coagulated 250 grs. of milk. 
