Eelenium  Autumnale.  22$ 
A  small  quantity  of  the  resinous  substance  was  next  subjected  to 
the  test  for  a  glucoside.  It  was  heated  for  fifteen  minutes  with  diluted 
H2S04  (one  part  of  acid  to  ten  parts  of  water),  then  rendered  alka- 
line by  the  addition  of  solution  of  NaHO,  and  a  few  drops  of  alka- 
line solution  of  CuS04  added,  and  the  whole  heated  to  the  boiling 
point,  whereby  a  precipitate  of  Cu20  was  formed,  proving  the  pres- 
ence of  glucose. 
The  substance  undissolved  by  the  70  per  cent,  alcohol  was  next 
treated  with  hot  90  per  cent,  alcohol,  which  dissolved  it  almost 
entirely,  leaving  only  slight  traces  of  fat.  The  alcohol,  upon  coolingr 
deposited  all  it  had  taken  up,  which  consisted  of  wax,  chlorophyll, 
and  other  coloring  matter ;  the  deposit,  after  thoroughly  washing 
with  alcohol  of  the  same  strength,  and  then  drying,  was  of  a  soft 
consistence,  having  a  grayish-green  color  and  a  bland  taste. 
The  aqueous  solution  of  the  ethereal  extract,  which  had  been  set 
aside  to  clear,  was  separated  from  a  slight  resinous  deposit,  concen- 
trated, filtered  and  set  aside  to  crystallize.  At  the  expiration  of  two 
days,  no  crystals  having  been  formed  in  the  liquid  during  this  timer 
it  was  treated  with  the  following  reagents :  NH4HO  produced  no 
change  except  deepening  the  color  of  the  liquid.  NH4HC03  had  the 
same  effect.  CaCl2  produced  no  change.  Fe2Cl6  produced  a  black 
coloration.  Gelatin  caused  a  turbidity.  Neither  Pb2C2H302  nor  tan- 
nic acid  effected  a  change  in  the  liquid. 
Treatment  with  Alcohol. — The  dregs,  after  having  been  exhausted 
with  stronger  ether,  were  freed  from  all  traces  of  ether  by  exposure 
to  the  air,  and  then  digested  in  95  per  cent,  alcohol  for  three  days, 
at  a  temperature  of  +150°  F.,  and  then  allowed  to  macerate  for  one 
day  longer  at  an  ordinary  temperature,  the  whole  thrown  into  a  perco- 
lator, and  the  dregs  thoroughly  exhausted  with  alcohol  of  the  same 
strength ;  the  washings  added  to  the  percolate,  and  the  whole  evapo- 
rated over  a  water  bath.  The  resulting  extract  was  treated  with  a 
small  quantity  of  distilled  water  at  a  gentle  heat,  the  whole  allowed 
to  cool,  after  which  the  solution  was  filtered  off,  the  residue  thoroughly 
washed  with  distilled  water,  the  washings  added  to  the  solution,  and 
the  whole  concentrated  to  one-half  its  bulk,  and  set  aside  in  a  cool 
place.  The  liquid  was  slightly  bitter,  having  a  faint  acid  reaction 
and  a  light-brown  color. 
The  portion  of  the  alcoholic  extract  insoluble  in  water  was  dried 
and  again  dissolved  in  alcohol,  treated  with  animal  charcoal,  filtered 
and  evaporated.    The  resulting  resin  was  of  a  dark-brown  color,  and 
