Am.  Jour.  Phabm  > 
May  1,  1874.  J 
Heleniam  Autumnale. 
225 
alcohol,  and  treated  with  H2S,  filtered,  and  then  evaporated,  which 
left  a  yellowish  coating,  which,  on  solution  and  application  of  Fe2Cl6 
and  gelatin,  proved  to  be  tannic  acid. 
Treatment  with  Cold  Water, — The  substance,  after  having  been  ex- 
hausted with  alcohol,  was  dried  and  then  macerated  with  cold  water,  t 
in  a  cool  place,  for  six  days,  and  strained,  which  yielded  a  turbid  . 
liquid  of  a  light-brown  color,  tasteless,  and  neutral  to  litmus.  On 
standing,  a  small  quantity  of  inulin  was  deposited.  The  clear  liquid 
was  poured  from  the  sediment,  and  heated  to  the  boiling  point,  where- 
by a  considerable  amount  of  albumen  was  separated,  which  was  fil- 
tered off,  and  the  filtrate  evaporated  to  one-half  its  bulk,  after  which 
the  following  reagents  were  applied  : 
NH4HO  and  Na2C03  deepened  the  color  of  the  solution.  Fe2Cl6 
produced  no  change.  Pb2C2H302  produced  a  slight  flocculent  precip- 
itate, soluble  in  HC2H302,  which  was  afterwards  proven  to  be  color- 
ing matter.  Trommer's  test  for  glucose  showed  its  absence  in  this 
solution. 
Treatment  with  Boiling  Water, — The  substance,  after  having  been 
exhausted  with  cold  water,  was  next  treated  with  boiling  water  for 
one  hour,  then  strained  and  concentrated  to  a  small  bulk,  and  sub- 
jected to  the  following  reagents  : 
NH4HO,  Na2C03,  KHO,  CaCl2,  Fe2Cl6  and  tannic  acid  produced 
no  change  in  the  liquid,  except  that  the  color  was  deepened  by  the 
alkalies.    Pb2C2H302  produced  a  copious  brown  precipitate. 
The  whole  of  the  liquid  was  then  treated  with  Pb2C2H302,  and  the 
precipitate  separated  by  a  filter  and  washed.  The  filtrate  and  wash- 
ings were  neutralized  with  NH4HO,  producing  a  slight  yellowish-white 
precipitate,  which  was  filtered  off  and  the  filtrate  treated  with 
Pb202C2H302  without  producing  any  further  change. 
The  Pb2C2H302  precipitate  was  then  treated  with  boiling  water, 
filtered,  and  the  filtrate  evaporated,  which  left  a  small  crystalline  resi- 
due, an  organic  acid  in  combination  with  lead,  apparently  malic  acid. 
The  portion  of  the  Pb2C2H302  precipitate  insoluble  in  boiling  water 
was  digested  in  diluted  HC2H302  and  filtered,  the  filtrate  treated  with 
NH4HO,  which  produced  no  precipitate,  showing  that  nothing  had 
been  dissolved  by  the  acid.  The  precipitate  was  then  boiled  with  a 
solution  of  NallO  and  filtered,  the  filtrate  tested  with  solution  of 
Ca2HO  for  oxalic  acid,  giving  a  negative  result. 
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