Am.  Jour.  Pharm.") 
January,  1896.  / 
North  American  Coniferce. 
37 
traces  were  removed  with  the  aid  of  a  water-bath,  and  the  residue 
of  the  leaves  was  then  treated  with  distilled  water.  This  solvent 
extracted  5-56  per  cent,  of  the  leaves.  Mucilage  and  glucose  were 
present  in  this  water  extract. 
Proximate  Analysis  of  the  Bark  of  the  Stem. — This  analysis  was 
conducted  as  in  the  case  of  the  leaves.  Absolute  alcohol  removed 
all  of  the  chlorophyll  and  other  substances  to  the  amount  of  29-25 
per  cent.  The  alcoholic  liquid  was  acid  to  litmus  paper.  From 
this  alcoholic  extract,  water  dissolved  8-65  per  cent,  of  the  bark,  and 
afforded  an  acid  solution  which  gave  the  following  reactions : 
Ferric  chloride,  dark  green  color  and  precipitate. 
Bromine  water,  heavy  yellow  precipitate. 
Calcium  hydrate,  little  change,  even  on  standing. 
Petroleum  ether,  applied  to  the  extract  after  the  latter  had  been 
treated  with  water,  dissolved  most  of  the  remaining  coloring  and 
waxy  substances,  but  left  undissolved  a  brown  residue,  consisting  of 
resinous  matter  and  the  principle  mentioned  under  the  leaves  as 
having  a  gritty  character. 
After  extraction  by  absolute  alcohol,  the  bark  produced  a  thick, 
mucilaginous  liquid  with  water.  The  liquid  gave  a  precipitate  when 
mixed  with  alcohol,  and  reduced  Fehling's  solution. 
Estimation  of  Tannin  in  Leaves. — The  hide  powder  method  was 
employed  in  this  and  in  all  other  estimations  of  tannin  stated  in  this 
paper.  There  is  reason  to  believe  that  the  hide  powder  absorbs 
some  of  the  acid  principle  as  it  does  the  tannin.  The  air-dry  leaves 
showed  2-18  per  cent,  of  tannin.  They  also  contained  15-21  per 
-cent,  of  moisture,  which,  if  allowed  for,  will  indicate  2-57  per  cent, 
of  tannin  in  leaves  which  are  absolutely  dry.  The  ash  calculated 
for  perfectly  dry  leaves  was  found  to  be  2*94  per  cent.  It  consisted 
mainly  of  calcium  phosphate  with  sulphate  and  carbonate,  and  a 
little  silica. 
Estimations  of  Tannin  in  the  Barks  of  Stem  and  Root.— These 
were  calculated  as  was  done  with  the  leaves,  and  the  following  chart 
is  employed  with  the  view  of  aiding  comparison  of  the  several  parts 
of  Pinus  Strobus : 
Ash  in 
Absolutely 
Tannin 
in  Moist 
Condition, 
Tannin  in 
Absolutely 
Moisture. 
Dry. 
2*94 
2-31 
4-67 
Dry. 
2 '57 
9 '35 
6-48 
Leaves  . 
Stem  bark 
Root  bark 
15-21 
9-10 
ii-i6 
2'l8 
8-50 
576 
