8o 
The  Assay  of  Cinchona  Bark. 
f  Am.  Jour.  Pharm. 
1  February  1896. 
ammonia  water,  and  an  aliquot  part  taken  for  analysis.  Schweis- 
singer-Sarnow,1  Keller2  and  the  author.3 
(2)  The  powdered  bark  or  its  preparations  are  macerated  with  a 
mixture  of  chloroform  or  ether,  or  a  mixture  of  both  in  conjunction 
with  alcohol  and  ammonia  water,  and  an  aliquot  part  taken  for 
analysis.  Prollius,4  De  Vrij,5  Lyons,6  Haubensak,7  Kursteiner8  and 
U.  S.  P.,  1890. 
The  table  below  is  given  to  show  at  a  glance  how  closely  some  of 
the  extractive  solvents  of  the  various  methods  approximate  one 
another : 
Tabular  View  of  the  Agents  Employed  in  Extracting  the  Alkaloids,  Etc. 
Process. 
Cinchona 
Bark. 
Alcohol. 
Chloroform 
Ether. 
NH4OH 
10  Per  cent. 
Amount  of 
Menstr. 
Taken  per 
Assay. 
Color  of 
Alkaloids. 
U.  S.  P.,  1890  .  .  . 
20  gms. 
152  cc 
40  c.c. 
8  c.c. 
100  c.c. 
Chocolate. 
20  gms. 
20  gms. 
170  gms. 
10  c.c. 
120  gms. 
Chocolate. 
Lyons  Nos.  1  and  21 
20  gms. 
13  8  cc 
180  7  c.c. 
5'55  c.c. 
TOO  c.c. 
( No.  1  chocolate. 
\  No.  2  nearly  white. 
L-yons  Nos.  1  and  2 2 
20  gms. 
137  c.c. 
43"9  c.c. 
137  c.c. 
5'45  c.c. 
100  c.c  ' 
/  No.  1  chocolate. 
1  No.  2  nearly  white. 
Haubensak  .... 
20  gms. 
20  c.c 
170  c.c. 
10  c.c. 
100  c.c. 
Nearly  white. 
Haubensak  and  \ 
Kiirsteiner3    .  .  j 
20  gms. 
30  gms. 
170  gms. 
15  c.c. 
100  gms. 
Nearly  white. 
Haubensak  and  ) 
Keller  j 
12  gms. 
120  gms. 
TO  c.c. 
100  gms.  I 
Yellowish  white. 
Chloroform-  ) 
Ether*  j" 
20  gms. 
50  gms. 
150  gms. 
20  C.C. 
ico  gms.  j 
f  Whiter  than  any 
\    other  process. 
1  Prollius'  mixture. 
2  Prollius'  fluid  modified. 
3  Moistens  the  drug  first  with  5  grammes  of  12"  19  per  cent,  hydrochloric  acid. 
4  The  method  as  used  by  the  writer  for  several  years  and  applied  to  the  various  drugs  and 
their  preparations.  Mr.  Keller  has  recently  published  a  process  for  assaying  cinchona  bark, 
that  approximates  this  one  very  closely.  Schweiz.  Wochenschr.  f.  Chem.  u  Pharm.,  through 
the  Pharm.  Era  (1896),  15,  78. 
5  Strong  ammonia  water. 
1  1890,  Pharm.  Centralhalle,  31,  771. 
2  1892,  Schweiz.  Wochenschr.  f.  Chem.  u.  Pharm.,  30,  501,  509;  Am.  J. 
Pharm.,  65,  78.  1893,  Ztschr.  Oesterreich.  Apotheker,  47,  563,  586  ;  Am.  J. 
Pharm.,  66,  42. 
3  1895,  Am.  J.  Pharm.,  67,  499  ;  J.  Am.  Chem.  Soc,  17,  822. 
4 1881,  Arch.  d.  Pharm  ,  209,  85,  572  ;  Am.  J.  Pharm.,  54,  59. 
5  1882,  Neder.  Tijdschr.  de  Pharm.,  January  ;  Am.  J.  Pharm.,  54,  290. 
6  1884,  Druggists'  Circular,  38,  114;  Pharmaceutical  Assay,  \  13,  14,  29-36, 
127,  128. 
7  189 r,  Schweiz.  Wochenschr.  f.  Pharm.,  29,  147  ;  Am.  J.  Pharm.,  63,  347- 
8  1892,  Schweiz.  Wochenschr.  f.  Chem.  u.  Pharm.,  30,  473  ;  Pharm.  Ztg.,  37, 
750  ;  Am.  J.  Pharm.,  i  5,  71. 
