240  Volatile  Oil  of  Ucvta  Metadata.  {A%JaTiXarm" 
No.  6 :  Heavy,  transparent ;  color,  dark  mahogany  ;  odor,  strongly 
empyreumatic. 
Nos.  I,  2  and  3  seem  to  be  the  most  important  fractions,  and  this 
treatise  has  to  do  mainly  with  the  consideration  of  their  proper- 
ties ;  however,  some  tests  were  made  on  some  of  the  other  frac- 
tions, and  these  will  be  mentioned  in  their  proper  places. 
Nos.  6  and  7  were  treated  with  alkali  in  order  to  ascertain  if  they 
were  esters,  but  with  negative  results. 
Strips  of  filter  paper,  moistened  with  potassium  iodide  solution 
and  starch  water  and  suspended  above  the  oil  in  the  tubes  contain- 
ing Nos.  1,  2  and  3,  rapidly  turned  purple,  showing  the  presence  of 
a  considerable  amount  of  ozone  in  the  air  confined  with  the  oil. 
The  corks  of  these  tubes  became  quite  white  in  color,  having  been 
subjected  to  the  bleaching  action  of  the  ozone  produced  by  the  oil. 
A  few  drops  of  each  of  the  fractions  were  placed  in  clean,  dry  test 
tubes,  and  enough  glacial  acetic  acid  added  to  each  to  effect  solu- 
tion. This  was  followed  in  each  case  by  the  addition  of  a  few  drops 
of  strong  sulphuric  acid.  The  following  phenomena  were  noticed  : 
No.  I  assumed  a  reddish-brown  color ;  Nos.  2,  3  and  4  became  dark 
purplish-red  ;  Nos.  5  and  6  became  dark  purple.  Upon  heating,  all 
became  nearly  black,  and,  upon  cooling,  became  denser  in  consist- 
ency, Nos.  3,  4,  5  and  6  becoming  quite  gelatinous.  Upon  standing 
for  forty-eight  hours,  Nos.  5  and  6  became  solid.  Upon  longer 
standing,  each  separated  into  two  layers,  the  bottom  layer  in  each 
case  being  of  a  dirty  brown  color,  and  the  upper  layer  of  a  pur- 
plish hue.  Of  the  fractions  themselves,  Nos.  1,  2,  3  and  4  each 
decolorized  an  ethereal  solution  of  bromine.  Nos.  1  and  2  each 
violently  decomposed  strong  nitric  acid,  and  also  reacted  with  a 
mixture  of  strong  nitric  acid  and  alcohol  with  almost  explosive  vio- 
lence. Nos.  1,  2,  3  and  4  showed  no  change  of  color  with  a  solu- 
tion of  ferric  chloride  in  absolute  alcohol,  but  No.  5  gave  a  dark  red 
color.  Nos.  I,  2  and  3  dissolved  iodine  quite  readily,  but  not  with 
violence,  Nos.  1  and  2  decolorizing  the  iodine  solution  upon  stand- 
ing for  some  time.  All  of  the  fractions  were  soluble  in  an  equal 
bulk  of  glacial  acetic  acid.  Nos.  I,  2  and  3  were  also  soluble  in 
equal  bulks  of  commercial  alcohol,  acetone  and  ether  ;  and  in  all 
proportions  in  benzin,  benzol,  chloroform  and  carbon  disulphide. 
They  were  but  slightly,  if  at  all,  soluble  in  glycerin.  As  most  of 
these  reactions  and  solubilities  correspond  with  those  of  the  class  of 
