^Alf;imarm'}            Assay  for  Sanguinaria.  307 
No.  2. 
Drug  (No.  50  powder)   10  grammes. 
Ether   iro  " 
Aqua  ammonise   10  " 
The  succeeding  steps  of  this  process  are  the  same  as  in  No.  1. 
No.  3. 
Drug  (No.  50  powder)   10  grammes. 
Prollius'  fluid  ;                           .  .  100  c.c. 
Lyons'  General  Assay  Process  No.  1,  taken  from  Pharmaceutical  Assaying. 
No.  4. 
Drug  (No.  50  powder)                     .  '   10  grammes. 
Petroleum  benzin   100  " 
Aqua  ammouiae    10  " 
Proceed  same  as  No.  1  and  No.  2. 
The  results  obtained  by  these  processes  were  as  follows : 
Process 
Used. 
Solvent  Used  for  Second  Extraction. 
Per  Cent,  of 
Alkaloid. 
Color. 
No.  1  ... 
4-86 
White. 
No.  2   .  . 
Ether   
5;  12 
White. 
No.  3   .  .  . 
6-o6 
Dark. 
No.  4  .  .  . 
1-48 
White. 
1  Heavy  chloroform  ether  consists  of  chloroform  3  volumes  and  ether  1  volume. 
The  low  results  obtained  in  No.  4  were  at  first  looked  upon  as 
being  incorrect,  but  a  repetition  of  the  process  afforded  the  same 
results. 
A  combination  of  processes  Nos.  1  and  4  was  then  tried.  This 
showed  that  the  benzin  exerted  a  solvent  action  upon  only  a  portion 
of  the  alkaloid  present,  and  gave  constant  results  with  the  same 
sample ;  a  subsequent  extraction  of  the  residue  with  light  chloro- 
form ether  removed  the  remainder  of  the  alkaloid,  and  gave  results 
for  total  extraction  agreeing  within  a  small  fraction  of  1  per  cent. 
A  sample  of  the  ground  drug  was  carefully  assayed,  using  the 
benzin  process,  and  contained  1-68  per  cent,  alkaloid.  A  fluid 
extract  was  then  prepared  from  the  same  drug,  using  the  process  in 
