Am'jJu°iy'il,96arm'}        Some  Recent  Sugar  Reactions.  365 
These  temperature  variations  are  taken  advantage  of  in  the  Worm- 
Mueller  modification. 
As  given  by  the  author,  the  test  is  applied  as  follows : 
Solutions  of  cupric  sulphate  of  2-5  per  cent.,  and  of  10  per  cent, 
of  potassium  and  sodium  tartrate  in  4  per  cent.  NaOH  or  5*6  per 
cent.  KOH,  are  prepared.  1  c.c.  of  the  copper  solution  is  mixed 
with  2-5  c.c.  of  the  alkaline  Rochelle  salt  solution.  5  c.c.  of  the 
urine  to  be  examined  are  employed. 
Both  liquids  are  heated  at  the  same  time  to  the  boiling  point,  the 
boiling  simultaneously  interrupted,  and  after  standing  20  to  25  sec- 
onds, no  sooner,  mixed  and  again  allowed  to  stand. 
The  temperature  of  the  liquids  at  the  point  of  mixing  is  stated  to 
be  about  8o°  to  85 0  C,  but  will  rapidly  fall  to  60  0  and  lower. 
According  to  the  statement  of  the  author,  this  procedure  will 
produce  the  same  effect  as  if  the  mixture  is  heated  to  6o°  or 
700  C. 
If  in  5  or  10  minutes  no  cuprous  oxide  is  precipitated,  the  test  is 
repeated  with  an  increase  of  0*5  c.c.  of  the  copper  solution,  the  alka- 
line mixture  remaining  as  before.  The  copper  solution  may  in  this 
manner  be  increased  until  about  4  c.c.  are  taken. 
If  glucose  is  present,  the  oxide  will  be  suspended  throughout  trie 
liquid  in  the  form  of  a  dirty  yellowish-green  cloud. 
A  phosphate  deposit,  which  may  possibly  obscure  small  amounts 
of  the  oxide,  will  soon  sink  to  the  bottom. 
The  author  states  that  0-025  percent,  of  glucose  may  be  detected 
in  the  urine  by  this  method. 
NOTES. 
A.  Jolles  (see  Proceedings  of  the  A.  Ph.  A.,  1895,  P-  7^o)  places 
the  delicacy  of  this  test  at  0  08  per  cent. 
In  a  number  ot  determinations  the  writer  has  obtained  results 
which  correspond  with  the  statement  of  Worm-Mueller  as  to  the 
percentage  found. 
But  in  several  instances,  while  examining  the  urine  of  gouty 
patients  or  of  those  passed  after  the  administration  of  piperazine, 
partial  reduction  of  the  test  solution  was  found  to  take  place. 
As  with  Fehling's  solution,  this  test  is  also  affected  byglycuronic 
urines. 
After  the  administration  of  creosote  or  sodium  salicylate,  the 
