378 
Morphine  in  Toxicological  Cases. 
Am.  Jour.  Pharin. 
July,  1896. 
gr.,  containing  10  per  cent,  tannin,  was  added,  and  the  whole  digested 
two  days,  at  400  C,  frequently  stirring.  The  mass  was  strained; 
the  residue  washed  with  a  fresh  quantity  of  glycerin,  containing  10 
per  cent,  tannin ;  again  strained  ;  this  operation  was  repeated  for  a 
third  time.  The  combined  glycerin  extracts  were  heated  to  500  C, 
cooled,  and  diluted  with  an  equal  volume  of  water.  In  an  experi- 
mental way  I  found  that  this  dilution  was  a  necessity  or  petroleum 
ether  would  not  separate  clearly.  Washed  with  petroleum  ether 
(boiling  point,  500).  Repeated  this  twice.  Removed  petroleum 
ether.  Warmed  glycerin  extract,  to  expel  traces  of  petroleum 
ether.  Cooled.  Washed  (the  extract  being  acid)  with  chloroform, 
removed  chloroform.  Now  added  chloroform,  containing  10  percent, 
alcohol.  Made  alkaline  with  NaOH.  Added  NaHCOs.  Agitated. 
Collected  the  chloroform.  Evaporated  it.  It  left  a  color  and 
odorless  residue,  proving  the  excellence  or  superiority  of  this 
method  as  compared  with  the  older  ones.  (Kippenberger,  Beitrage 
zur  Reinisolirung,  quant.  Trennung  u.  chem.  Characteristik  v.  Alka- 
loiden  u.  glycosidartigen  Kbrpern,  in  forensen  Fallen.  Wiesbaden. 
C.  W.  Kreidel.  1895.) 
VIII.  Good  results  of  the  methods  of  Dragendorff,  Stas-Otto, 
Graham  and  Kippenberger,  above  referred  to. 
Applied  the  sulphomolybdate  test  (Frbhde's) ;  obtained  a  most 
positive,  decided  and  strong  morphine  reaction.  The  purple  color 
appeared  immediately. 
Applied  the  ferric  chloride  test.  One  drop  of  the  ferric  chloride 
solution  added  to  a  residue  of  evaporation  of  a  portion  of  the 
isobutylic  alcohol  caused  the  characteristic  blue  color  reaction. 
Applied  the  H2S04  and  HNOs  test  (Husemann's).  A  very  dis- 
tinct blood-red  color  appeared,  where  the  glass  rod,  previously 
moistened  with  HNOs,  touched  the  H2S04. 
Applied  the  KI03  test.  The  previously  colorless  mixture  of 
KI03,  acetic  acid  and  water  became  immediately  brownish-yellow, 
upon  the  addition  to  the  mixture  of  the  solution  in  acetic  acid  of 
a  residue  of  evaporation  of  another  portion  of  isobutylic  alcohol. 
After  all  these  satisfactory  tests,  half  of  the  isobutylic  alcohol, 
that  was  left,  being  colorless,  was  evaporated.  Left  a  bad-smelling, 
amorphous  residue.  A.  (Proof  for  the  accuracy  of  Tamba's  and 
Graebener's  statements.)  This  residue  was  heated  for  an  hour  to 
1000   C;   taken  up  with  a  small  quantity  of  acidulated  water, 
