Am'jJu°iy1!'i896arm'}    Acetanilid  in  Synthetical  Remedies.  39 r 
an  excess  of  bromine  water  had  been  added,  when  the  turbidity  or 
precipitate  became  permanent.  Exalgin  in  this  test  separated 
yellow  oily  drops.  The  tests  were  then  modified,  bromine  water 
being  added,  drop  by  drop,  during  a  period  of  five  minutes,  as  fast 
as  the  color  was  discharged. 
Acetanilid  . 
Exalgin 
Methacetin 
Phenacetin 
Phenocoll  . 
Iyactophenin 
Salophen  . 
c.c.  Bromine 
Water  Added.         Appearance  after  Stirring  Five  Minutes. 
2-o  Yellow  liquid;  white  ppt. 
o'8  Colorless  liquid;  white  ppt. 
i*8  c  Yellow  liquid,  becoming  colorless, 
o-6  \  pink,  finally  red-brown,  slightly 
2'o        v  turbid. 
1  "2  Liquid  like  above;  distinct  white  ppt. 
o*i  Colorless,  slightly  turbid  liquid. 
By  agitating  the  tests  afterwards  with  a  light  petroleum-benzin  it 
was  found  that  the  exalgin  precipitate  was  readily  soluble  (yielding 
beautiful  white  crystals  upon  the  evaporation  of  the  benzin),  the 
acetanilid  precipitate  slightly  soluble  (yielding  a  small  quantity  of 
crystals  upon  the  evaporation  of  the  benzin),  while  the  lactophenin 
precipitate  was  insoluble  ;  ethe.r  does  not  give  such  satisfactory  re- 
sults, as  it  takes  up  all  of  the  precipitates  besides  coloring  sub- 
stances produced  in  the  other  tests.  The  bromine  test,  therefore, 
gave  hopes  of  being  applicable  to  the  detection  of  acetanilid  in  alL 
of  the  remedies  excepting  lactophenin  ;  it,  hence,  was  of  importance 
to  determine  its  sensitiveness  with  acetanilid  solutions.  With 
I  :  2,000  solutions  in  distilled  water  the  precipitate  with  bromine 
water  could  always  be  obtained  after  stirring  for  several  minutes  ; 
solutions  of  greater  dilution,  1  :  10,000,  were  not  certain  to  yield  the 
precipitate,  even  after  prolonged  standing  or  stirring.  The  test, 
therefore,  should  be  practical  in  solutions  containing  not  less  than 
1  :  2,000;  this  calculated  to  01  gm.  of  any  of  the  remedies  with  10 
c.c.  water,  would  be  equivalent  to  indicating  5  per  cent,  acetanilid — 
the  claim  made  by  Hirschsohn.  To  substantiate  this  and  test  its 
greater  applicability,  o- 1  gm.  of  each  of  the  remedies  were  heated 
with  10  c.c.  of  a  1  :  2,000  acetanilid  solution,  the  solution  cooled,  fil- 
tered and  5  c.c.  of  the  filtrate  used  as  before  ;  the  results,  excepting 
with  salophen  and  exalgin,  were  disappointing,  as  it  was  not  possible 
to  certainly  distinguish  between  the  tests  made  with  distilled  water 
or  with  the  acetanilid  solutions.  After  trying  the  effect  of  varying 
quantities  of  bromine  water,  added  slowly  or  in  one  portion  to  so- 
