'^"^  rtr'^isss*^™'}        Analysis  of  Fouquieria  Splendens.  85 
insoluble  in  petroleum  spirit,  slightly  soluble  in  95  per  cent,  alcohol 
and  carbon  disulphide,  quite  soluble  in  cold  absolute  alcohol,  amyl 
alcohol,  chloroform,  benzol  and  oil  of  turpentine.  Nitric  acid  gave 
no  reaction.  With  sulphuric  acid  and  small  portions  of  the  ethereal 
residue,  I  obtained  a  dark  mahogany  color.  This  solution  on  adding 
absolute  alcohol  was  partially  discolored,  no  precipitate.  With  ether 
the  sulphuric  acid  solution  gave  a  greenish  precipitate,  with  amyl 
alcohol  the  acid  solution  was  discolored  changing  to  pale  red,  then 
green.  These  tests  show  in  each  case  a  wide  difference  in  color  re- 
actions from  those  obtained  with  the  petroleum  spirit  residue.  The 
amount  of  solids  taken  up  on  treating  the  ethereal  residue  with  water 
was  '36  per.  cent.  The  aqueous  liquid  was  neutral  to  litmus,  portions 
tested  for  alkaloids  gave  negative  results ;  on  warming  and  addition  of 
dilute  sulphuric  acid,  Fehling's  solution  was  reduced,  indicating  possibly 
glucosides.  The  portion  insoluble  in  water  was  then  treated  with 
absolute  alcohol.  The  liquid  gave  an  acid  reaction  with  test  paper.  A 
measured  part  of  the  liquid  was  evaporated  and  the  weighed  residue 
showed  1*6  per  cent,  of  solids  dissolved.  The  residue  from  the  evapo- 
rated alcoholic  liquid  was  partially  dissolved  by  aqueous  alkalies.  It 
readily  saponified  with  alcoholic  soda,  forming  a  soft  brown  soap, 
which  on  boiling  with  lead  acetate  yielded  a  yellow  precipitate.  This 
was  collected  on  a  filter  and  washed.  When  the  precipitate  was  boiled 
with  absolute  ether  and  the  filtrate  allowed  to  slowly  evaporate,  a 
white  organic  crystaline  substance  separated  out.  Under  the  microscope 
particles  of  coloring  matter  were  found  to  be  interspersed  among  the 
crystalline  structures. 
The  indications  would  show  an  acid  resin  to  have  been  extracted  by 
the  ether. 
The  ten  grammes  of  powdered  bark,  after  exhaustion  with  petroleum 
spirit  followed  by  absolute  ether,  were  treated  with  absolute  alcohol. 
A  measured  quantity  of  the  alcoholic  extract  was  evaporated  in  a 
weighed  platinum  dish,  dried  until  weight  noted  was  constant.  After 
incineration  the  amount  of  ash  was  found  to  be  '15  per  cent,  of  the 
original  material.  The  alcoholic  extract  for  determination  of  total 
amount  of  organic  solids  dissolved,  was  evaporated  in  a  current  of  car- 
bonic acid,  when  the  residue  dried  gave  8*6  per  cent,  and  7*98  per  cent, 
of  solids  respectively.  A  cloudiness  formed  on  the  addition  of  water 
to  the  residue,  which  cleared  up  on  addition  of  alkalies.  It  was 
Tostored  by  acid.    The  aqueous  liquid  gave  precipitates  with  calcium 
