Am.  Jour.  Pharm. 
Oct.,  1885. 
Gleanings  from  Foreign  Journals. 
503 
GLEANINGS  FROM  FOREIGN  JOURNALS. 
By  J.  Robert  Moechel. 
Detection  of  Blood  in  Urine. — A  new,  sure  and  simple  method  by 
means  of  which  the  pecuh'ar  color  of  blood  is  obtained  is  given  by 
A  ntonio  Luchini  in  L'Orosi.''  To  10  cc.  of  urine,  in  a  test-glass, 
add  one  drop  of  acetic  acid  and  3  cc.  of  chloroform,  and  shake  well. 
In  the  presence  of  blood,  the  subsiding  chloroform  will  show  more  or 
less  of  the  red  blood-tint,  according  to  the  quantity  of  blood  present. 
Luchini  experimented  with  personally  prepared  solutions  of  blood, 
and  observed  the  reaction  to  be  successful  with  a  solution  containing 
3  drops  of  blood  in  250  cc.  of  water. — Schweiz.  Wochenschrift  f.  Ph., 
188e5,  p.  220. 
Diphenylamine.,  (C6H5)2NH,  is  a  very  delicate  reagent  for  the  detec- 
tion of  nitrogen  acids  and  for  free  chlorine.  H.  Hager  recommends 
two  solutions  as  being  convenient  for  use:  1,  1  gram  of  dipheny la- 
mine  in  30  cc.  of  absolute  alcohol,  and,  2,  a  mixture  of  1  volume  of 
this  solution  with  5  or  6  volumes  of  pure  sulphuric  acid,  as  dipheny  1- 
.amine  sulphate.  Both  solutions  are  yellowish,  and  the  latter  becomes 
blue  in  the  presence  of  nitrogen  acids  and  other  oxidizing  agents. 
For  the  detection  of  chlorine,  place  3  or  4  cc.  of  the  suspected  liquid 
in  a  test-tube  of  1  cm.  width  and  pour  1  to  1*5  cc.  of  sulphate  of 
diphenylamine  carefully  down  the  side  of  the  tube,  so  as  to  let  it  col- 
lect on  the  bottom.  According  to  the  quantity  of  chlorine  present,  a 
blue  coloration  will  be  observed  between  the  layers  of  the  two  liquids, 
or  the  whole  of  the  lower  liquid  will  be  colored  blue.  Very  slight 
traces  may  be  detected  if  to  3  or  4  cc.  of  liquid  1  to  5  cc.  of  diphenyl- 
amine sulphate  be  added  with  the  care  stated  above,  so  as  to  have  two 
layers.  Place  the  tube  upon  a  sheet  of  white  paper  and  shake  well ; 
a  transient  blue  coloration,  rapidly  vanishing,  will  be  observed  ;  some- 
what larger  traces  of  chlorine  show  the  blue  color  for  a  longer  time.— 
Ph.  Centralkalle,  1885,  pp.  277-279. 
Naphtholy  C10H7OH,  is  a  delicate  reagent  for  the  detection  of  nitrates 
-and  the  nitrogen  acids,  though  not  as  delicate  as  diphenylamine. 
Hager  succeeded  in  detecting  1  in  5,000  and  obtained  a  fainter,  but 
still  distinct  reaction  with  1  in  8,000  parts.  The  solution  for  use  con- 
tains 1  part  of  naphthol  in  100  parts  of  strong  or  absolute  alcohol. 
Por  applying  the  test,  place  in  a  test-tube,  of  1'2  cm.  width,  3  or  4  cc. 
