530  Analysis  of  StilUngia  Sylvatica.  {^'"Novjisst"'"' 
duced  a  green  color  with  iron  salts,  and  white  precipitates  with  solu- 
tions of  tartar  emetic  and  morphine. 
The  filtrate  recovered  from  the  tannin  estimate  was  acidulated  with 
H2SO4,  mixed  with  an  equal  volume  of  alcohol,  filtered,  evaporated 
free  of  all  alcohol,  and  the  acid  solution  tested  for  alkaloids  and  glu- 
cosides,  with  results  showing  the  latter  to  be  absent.  With  phospho- 
molybdic  acid,  solution  of  platinic  chloride  and  Mayer's  solution  pre- 
cipitates were  obtained.  The  remaining  acid  solution  was  then  care- 
fully neutralized  with  ammonia,  and  the  resulting  precipitate  treated 
with  95  per  cent,  alcohol,  which  upon  evaporation  yielded  an  amor- 
phous powder.  After  several  unsuccessful  trials  to  obtain  an  addi- 
tional amount  of  the  alkaloid  the  following  plan  was  devised  :  The 
powdered  drug  was  mixed  with  one-third  of  its  weight  of  slaked  lime 
and  dried ;  the  mixture  was  treated  with  alcohol  until  exhausted  ; 
dilute  sulphuric  acid  was  added,  the  liquid  filtered,  evaporated  free 
from  alcohol,  and  on  neutralizing  the  acid  solution  the  alkaloid  was 
obtained.  When  heated  it  was  entirely  volatilized;  treated  with 
KHO,  ammonia  evolved ;  with  HgSO^  it  combines  to  form  a  sulphate, 
which  was  obtained  in  fine  scale-like  crystals.  For  this  alkaloid  I 
propose  the  name  of  Stillingine. 
Cold  Water  Extract. — The  drug,  after  the  alcohol  treatment,  was 
macerated  and  percolated  with  cold  water  until  exhausted ;  the  perco- 
late was  of  a  straw-yellow  color,  yielded  an  extract  weighing  0*55  Gm., 
or  2*75  per  cent.  It  was  found  to  be  principally  gum.  Strong  alcohol 
and  solution  of  subacetate  of  lead  produced  copious  precipitates. 
Acid  Extract. — After  drying  the  residue  of  the  foregoing  operation 
it  was  found  to  weigh  10  648  Gm.  It  was  mixed  with  400  cc.  of 
water  and  10  cc.  of  HoSO^,  l^oiled  continually  for  eight  Lours,  water 
being  occasionally  added  to  preserve  the  quantity.  The  liquid  now 
contained  all  the  starch  of  the  root  as  glucose;  it  was  thrown  upon  a 
filter,  and  thoroughly  washed  with  warm  water  until  the  filtrate  mea- 
sured 8^0  cc.  Fehling's  solution  showed  the  presence  of  4*3243  Gm. 
of  glucose,  which  was  formed  from  3*89187  Gm.  of  starch.  The 
weight  of  the  insoluble  portion  after  drying  was  5*902  Gm.;  total  acid 
extract,  therefore,  4*746  Gm.,  and  of  acid  extract,  not  starch,  0*86413 
Gm.,  making  the  total  acid  extract  23*73  per  cent. 
Alkali  Extract. — The  above  remainder  was  boiled  for  3  hours  in 
200  cc.  of  a  10  per  cent,  solution  of  ^^aOH,  filtered,  and  washed  with 
water  to  remove  all  alkali ;  the  residue  after  drying  weighed  4*592 
