Mar'ch^Y92'S:}  Notes  OH  Soy  Beau  Urease.  155. 
TABI.E  I. 
Seed  No.  162,806.  o.i  Gm.  powdered  seed,  15  Cc.  H2O,  10  Cc.  i  per  cent, 
urea  solution,  temperature  40°. 
Time  in 
Time  in 
minutes. 
Cc.  iV/10  HCl. 
minutes. 
Cc.  iV/10  HCl. 
:  5 
3.6 
70 
26.3 
10 
6.7 
80 
28.5 
20 
10.9 
90 
30.2 
0 
14.8 
100 
31.6 
40 
18.2 
no 
32.7 
50 
21.3 
120 
33-2 
60 
24.2 
130 
33-2 
The  theory  for  10  Cc.  of  i  per  cent,  urea  is  33.3  Cc.  The  reaction 
was  therefore  quantitative  in  two  hours  under  the  conditions  of  the 
experiment. 
It  remained  to  be  determined  whether  the  method  of  prepara- 
tion of  the  sample  by  grinding  and  sieving  was  sufficiently  uniform 
for  comparative  tests.  Each  of  the  preparations  used  in  the  follow- 
ing experiment  was  ground  separately. 
Table  II. 
0.1  Gm.  powdered  seed,  15  Cc.  H2O,  10  Cc.  i  per  cent,  urea  solution,  tem- 
perature 40°,  time  30  minutes. 
vSeed  No.  Co.  AT/ 10  HCl.  vSeed  No.  Cc.  iV/lO  HCl. 
9  163115  II-4 
162804  15 
162804  15 
162804  15 
162806  14 
162806  14 
162806  14 
162806  14 
6  163115  II. I 
9  163115  II-3 
4  163115  II. 3 
5 
4 
It  is  evident  from  the  above  that  the  method  of  preparation  used 
is  applicable  for  purposes  of  comparison,  the  greatest  variation  ob- 
served being  0.4  Cc.  for  different  preparations  of  the  same  sample. 
As  might  be  expected,  the  velocity  of  the  reaction  increases  with 
rise  in  temperature,  as  shown  in  the  following  experiment. 
Table  III. 
Seed  No.  162,902.  o.i  Gm.  powder,  15  Cc.  H2O,  i  Cc.  i  per  cent,  urea, 
time,  30  minutes. 
Temperature.  Cc.  iV/10  HCl.  Temperature.  Cc.  iV/10  HCl. 
20          -                5,2                                    50  20.8 
30                          8.5                                    60  25.0 
40  14.9 
