^''May^^9^:}    Pkamiacology  of  Cotton  Root  Bark.  289 
vary  in  their  resistance  to  drugs  and  occasionally,  although 
rather  rarely,  one  will  find  combs  which  do  not  become  dis- 
tinctly blue  after  ergot,  the  effect  being  more  of  a  blanching. 
These  control  tests  shewed  that  the  combs  could  be  blued  by 
small  to  medium  doses  of  ergot. 
TABLE  2.    COCK'S  COMB  TESTS  ON  COTTON  ROOT  BARK. 
A  fluidextract  of  commercial,  dried  cotton  root  bark,  made 
with  a  menstruum  of  alcohol  and  glycerin  (3  to  1)  ,  gave  the 
following  results  on  the  fowls  previously  tested  with  ergot: 
Fowl 
Weight  in  Kg. 
Dose  per  Kg. 
Results 
394 
1.545 
1.0  c.c. 
No  noticeable  bluing  of  comb 
386 
1.544 
1.0  c.c. 
u 
u        u  u 
388 
1.460 
1.5  c.c. 
a 
u 
u        u  u 
393 
1.388 
2.0  c.c. 
u 
u 
u        u  u 
397 
1.665 
2.0  c.c. 
« 
u 
((         u  u 
384 
1.705 
2.5  c.c. 
u 
u 
u       u  u 
388 
1.467 
2.5  c.c. 
a 
a 
u       u  u 
391 
1.661 
3.0  c.c. 
u 
u 
tl          (I  u 
396 
1.706 
3.0  c.c. 
u 
u 
u        u  u 
398 
1.690 
3.0  c.c. 
(I 
u 
u        u  u 
400 
1.507 
3.0  c.c. 
u 
u 
((         u  u 
501 
1.644 
3.0  c.c. 
Very  faint  bluing  of  comb 
502 
1.553 
4.0  c.c. 
Extremely  faint  bluing  of  comb 
503 
1.532 
5.0  c.c. 
Faint  bluing  of  comb 
A  fluidextract  of  commercial,  "green"  cotton  root  bark, 
made  with  a  menstruum  of  alcohol  and  glycerin  (3  to  1) ,  gave 
results  as  follows : 
Fowl 
Weight  in  Kg. 
Dose  per  Kg. 
Results 
511 
1.703 
1.0  c.c. 
No  noticeable  bluing  of  comb 
515 
1.532 
1.0  c.c. 
u 
u 
«  u 
u 
512 
1.586 
2.0  c.c. 
u 
u 
u  u 
u 
516 
1.688 
2.0  c.c. 
u 
u 
((  a 
u 
530 
1.630 
2.0  c.c. 
u 
u 
u  u 
u 
514 
1.441 
3.0  c.c. 
u 
u 
u  u 
u 
517 
1.337 
3.0  c.c. 
u 
u 
u  u 
u 
524 
1.498 
3.0  c.c. 
u 
u 
u  « 
u 
518 
1.576 
3.0  c.c. 
u 
u 
u  u 
u 
511 
1.635 
3.0  c.c. 
Ci 
u 
u  u 
u 
512 
1.578 
3.0  c.c. 
u 
u 
515 
1.528 
3.0  c.c. 
u 
u 
u  u 
u 
516 
1.662 
3.0  c.c. 
il 
u  u 
u 
385 
1.715 
3.0  c.c. 
Extremely  faint  bluing  of  comb 
The  commercial  drug  did  not,  in  any  case,  produce  bluing 
of  the  cock's  comb  in  doses  smaller  than  3  c.c.  per  Kg.  Ths 
dose  of  ergot  required  for  these  same  fowls  was  from  0.50  to 
1  c.c.  Other  symptoms  were  evident,  however,  such  as 
blanching  of  the  comb,  wattles,  and  areas  about  the  eyes, 
drooping  of  the  feathers,  dyspnoea  and  diarrhoea.  With  doses 
of  4  and  5  c.c.  per  Kg.  the  fowls  seemed  very  sick,  and  while 
slight  bluing  was  produced,  the  end  point  was  so  indefinite 
that  a  quantitative  assay  by  this  method  seemed  to  be  quite 
impossible.   Doses  of  2  and  3  c.c.  per  Kg.  of  each  of  the  thir^ 
