Am.  Jour.  Pharm. 
October,  1920. 
Action  of  Adonis  Vernalis. 
705 
Tracing  i-D.  Showing  the  one  to  two 
rythms  often  displayed  after  the  appHca- 
tion  of  adonis.  The  time  is  marked  in 
seconds,  the  lever  moves  upwards  in  sys- 
tole and  downwards  in  diastole. 
dilated  and  filled  with  dark  unoxygenated  blood.  In  short  the 
hearts  appear  to  be  typical  digitalis  hearts. 
Since  the  frog  hearts  which  had  been  poisoned  with  adonis,  pre- 
sented the  typical  digitalis  picture,  it  seemed  reasonable  that  adonis 
could  be  assayed  by  the  one-hour  frog  method  which  is  official  for 
digitalis. 
The  information  with  regard  to  adonis  has  been  derived  from 
work  done  upon  two  lots  of  the  drug,  which  we  have  called  Drug  A 
and  Drug  B.  Alcoholic  tinctures  were  made  from  these  drugs  at 
different  times  and  they  were  assayed  as  follows: 
Drug  A. 
Per  cent,  of  alcohol  in  the    Toxicity,  or  the  minimum  systolic  dose 
THE  ASSAY  OF  ADONIS. 
menstruum  used. 
(M.  S.  D.)  per  gram  of  frog. 
95  0.0043  cc. 
95  0.0043  cc. 
80  0.0033  cc. 
70  0.0033  cc. 
60  0.0040  cc. 
50  0.0027  cc. 
This  makes  the  average  toxicity  0.0036  cc.  per  gram  of  frog. 
