473 
Titration  as  a  Means  of  Assaying  Drugs.  {Am  £™T- 
Phann. 
1893. 
Fluid  Extract. 
Aconite  Root, 
Bellad.  Leaves, 
Bellad.  Root,  . 
Method  of  Lyons. 
See  Lyons  Manual,  \  92. 
Method  of 
Lloyd.  + 
Method  of      Method  of 
Thompson. A  Beckurts.AA 
Chlorof.  ether. 
Bloodroot,  .  . 
Cinchona,  .  . 
Coca  Leaves,  . 
Colchicum  Seed 
Conium  Fruit, 
Gelsemium,  .  . 
Henbane,     .  . 
Ipecac,  .... 
Jaborandi,    .  . 
Nux  Vomica,  . 
Stramon.  Seed, 
Veratr.  Viride, 
120. 
g  120. 
I  127, 
4  154-** 
I  173- 
I  188.  t 
I  207. 
I  120. 
\  29. 
%  120. 
I  261. 
\  I20.1[ 
Ether  alone. 
Chlorof.  ether. 
0 
-  00 
+  Wherever  there  is  a  dash  the 
regular  method  of  Prof.  Uoyd 
using-  his  dried  soda  ferric  hy- 
drate mixture  and  plain  chloro- 
form has  been  employed.  The 
chloroform  ether  mixture  consist- 
ed of  equal  parts  of  each. 
*  Evaporate  F.  E.  Bloodroot  with 
HC1  and  water  to  remove  all  the 
alcohol.  Precipitate  with  ammo- 
nia and  filter.  Dissolve  precipi- 
tate in  dilute HC1  and  filter  again. 
Make  alkaline  and  extract  with 
ether. 
Benzine  70 ) 
.  Ether     25 / 
was  used  instead  of  benzine  alone. 
A  mixture  of 
t  Instead  of  titrating  with  so- 
dium phosphomolybdate  solution 
as  given  in  \  188  we  made  alkaline 
with  potassium  carbonate  and  ex- 
tracted with  benzine  and  evapor- 
ated in  tared  beaker  after  adding 
a  few  drops  of  dilute  hydrochloric 
acid. 
\  We  used  dilute  acetic  instead 
of  sulphuric  acid. 
O  The  chloroform  ether  extract 
was  allowed  to  evaporate  sponta- 
neously after  adding  some  dil.  HC1. 
Filtered  and  washed  with  dilute 
HC1  and  extracted  with  ammoni- 
ated  benzene-coloroform. 
||  Proceeded  as  under  O  but  ex- 
tracted with  chloroform  ether  fi- 
nally, making  extract  slightly  acid 
by  means  of  decinormal  hydro- 
chloric acid  and  heated  to  ioo°  C. 
weighing  as  coniine  hydrochlor- 
ide. 
00  Proceeded  as  under  0 
using  chloroform  ether  for  final 
extracting— allowed  this  to  evap- 
orate spontaneously  and  when  dry 
heated  to  ioo°  and  weighed, 
1  I  Chloroform  extract  is  evapor- 
ated at  moderate  heat  on  water- 
bath  ;  dilute  acetic  acid  is  then 
added  and  some  ether  to  insure 
combination  of  alkaloid  with  acid. 
After  evaporating  the  ether,  filter, 
wash,  and  then  make  alkaline 
with  ammonia  and  extract  with 
chloroform.  Evaporate  at  moder- 
ate heat  and  finallv  at  ioo°  C. 
A  Thompson's  Method  —  see 
Proceedings  of  the  Michigan  State 
Pharmaceutical  Association,  1891, 
p.  67. 
A  A  Beckurts'  Method  —  see 
Pharmaceutische  Rundschau, Vol. 
IX,  p.  255  (November,  1891). 
Baltimore,  June  28,  18 gj. 
