104 
Tincture  Deposits. 
Am.  Jour.  Pharnu 
Feb.,  1884. 
for  quinia.  1st.  By  dissolving  a  little  in  dilute  H2S04,  adding  bromine 
water  and  then  amnionic  hydrate.  A  fine  green  coloration,  due  to 
thalleioqitin  appeared.  2d.  The  remaining  alkaloid  was  dissolved  in 
a  small  quantity  of  hot  dilute  H2S04,  exactly  neutralized  with  amnionic 
hydrate,  still  being  kept  hot,  and  set  aside  for  a  short  time.  An 
abundant  supply  of  crystals  appeared,  showing  the  presence  of  quinia. 
Second  sample. — This  sample  was  much  lighter  in  color  than  the  other 
two,  and  far  larger  in  proportion  to  the  amount  of  tincture  from  which 
it  separated.  It  was  first  examined  quantitatively  for  alkaloids  and 
for  quinia.  7*237  grams  were  treated  as  above,  the  extraction  with 
chloroform  being  performed  in  a  "  Dunstan  and  Short's  apparatus  for 
continual  extraction/'  which  I  find  by  far  the  best  for  the  purpose- 
After  evaporation  of  the  chloroformic  solution  the  residue  was  dissolved 
in  dilute  II2S04,  heated  upon  a  water-bath,  exactly  neutralized  by 
AmHO  and  set  aside  to  crystallize,  filtered,  well  drained,  and  again 
dissolved  in  boiling  water,  set  aside,  the  crystals  collected,  drained  , 
dried  at  100°C.  and  weighed.  0*156  of  a  gram  was  obtained,  to  which 
was  added  0*0876  of  a  gram,  being  the  amount  of  quinia  sulphate 
retained  by  the  mother  liquors,  equivalent  to  2*94  per  cent,  of  quiniar 
or  3*204  per  cent,  of  crystalline  sulphate  of  quinine.  The  mother 
liquors  were  now  precipitated  by  ammonia  and  the  alkaloids  taken  up 
by  chloroform,  which  solution,  on  evaporation,  yielded  *3182  of  a  gram 
of  alkaloids,  from  which  was  deducted  *0697  of  a  gram  (the  quinia 
present)  leaving  *2485  of  a  gram  of  alkaloids,  not  quinia,  or  3*433  per 
cent. 
The  deposit,  therefore,  contains — 
Total  alkaloids  ,   6-374  per  cent. 
Quinia   2.940  per  cent. 
Cinchotannic  acid  was  proved  by  distilling  the  dry  deposit,  when  an 
odor  resembling  carbolic  acid  was  developed,  and  the  distillate  when 
tested  with  bromine  water  gave  a  white  precipitate  which  rose  to  the 
surface  of  the  liquid  (tribromophenol)  ;  and  by  the  slightly  acid  extract 
giving  a  faint  greenish  coloration  with  ferric  chloride.  Cinchona-red 
was  proved  as  below. 
Third  sample. — This  was  of  a  very  dark  color,  having  the  appear- 
ance of  cinchona-red.  1*35  gram  was  treated  as  before  for  alkaloids. 
The  yield  was  only  *034  of  a  gram  or  2*52  per  cent.  The  solution 
was  only  faintly  fluorescent.    On  attempting  to  crystallize  out  the 
