Am.  Jour.  Pharm. 
July,  1884. 
Analysis  of  Barley. 
367 
drawn  through  to  displace  remaining  gas.  The  acid  solution  was  now 
taken  out  of  the  bulbs  and  the  latter  thoroughly  rinsed,  the  water 
used  being  added  to  the  solution.  A  few  drops  of  solution  of  litmus 
were  added  and  the  liquid  carefully  neutralized  with  No.  II.  Of 
500  Cc.  of  No.  II  required  to  neutralize  10  Cc.  of  No.  I,  148*5  Cc. 
had  been  replaced  by  the  evolved  ammonia ;  in  other  words,  2*97  Cc. 
No.  I  were  neutralized  before  any  of  No.  II  had  been  added.  2*97 
multiplied  by  0*014  equals  0*04158  Gm.  nitrogen  in  2  Gm.  barley. 
This,  multiplied  by  6*25  yields  in  2  Gm.  of  barley  0*25987  Gm.,  or 
12*99  per  cent,  of  proteids. 
Benzin  Extract. — 10  Gm.  of  finely  powdered  barley  placed  in  a 
closely  covered  percolator,  were  macerated  with  30  Cc.  benzin  for  four 
hours.  It  was  then  allowed  to  percolate,  and  enough  benzin  was 
added  to  displace  the  quantity  retained  by  the  drug.  The  process  was 
repeated  three  times  and  insured  complete  exhaustion  of  the  drug,  as, 
when  a  few  drops  of  the  last  percolate  were  evaporated  on  platinum 
foil,  no  residue  was  obtained.  This  extract  was  evaporated  on  a 
water-bath  until  all  of  the  benzin  was  volatilized.  Weight  of  the 
extract  0*494  Gm.  A  little  water  was  added  to  the  extract  and  this 
was  again  evaporated  on  a  water-bath,  whereby  a  loss  of  0*069  Gm. 
was  incurred  which  was  ascribed  to  volatile  oil.  Of  the  remainder  of 
the  extract,  water  dissolved  0*03  Gm.  having  an  acid  reaction ;  alco- 
hol dissolved  0*245  Gm.  of  which  0*145  Gm.  was  soluble  in  alkali 
solution  and  precipitated  again  on  addition  of  mineral  acids ;  the 
portion  insoluble  in  the  alkali  was  an  oily  liquid  and  floated  on  water ; 
0*15  Gm.  of  the  extract  was  insoluble  in  water  or  alcohol.  The 
portion  soluble  in  alcohol  is  also  soluble  in  ether,  chloroform  and 
disulphide  of  carbon. 
Alcohol  Extract. — The  barley  treated  with  benzin  was  dried  at  a 
moderate  heat  and  exhausted  with  sufficient  (80  per  cent.)  alcohol  to 
make  200  Cc.  of  percolate,  which  had  an  acid  reaction.  20  Cc.  were 
used  for  finding  the  total  extract  — 0*5  Gm. —  and  ash  — 0*04  Gm. — 
Of  this  extract  — 0*36  Gm. —  was  soluble  in  water.  The  insoluble 
portion  — 0*14  Gm.  was  soluble  in  fixed  alkalies,  and  consisted  of 
proteids.  40  Cc.  were  evaporated  to  dryness  on  a  water-bath ;  the 
residue  was  exhausted  with  boiling  water  and  diluted  to  40  Cc.  This 
solution  boiled  with  Fehling's  solution  reduced  it,  showing  that  sugar 
or  an  allied  body  was  present. 
120  Cc.  were  carefully  neutralized  with  sodium  hydrate  and  evapo- 
