Aj;nuarryrSm'}       Substitute  for  Ethyl  Alcohol.  7 
was  101-43  grammes.  These  weights  added  to  the  differences  of 
the  table  give  the  weights  of  the  fractions  of  percolate. 
RATE  AND  DEGREE  OF  EXHAUSTION  BY  DIFFERENCES. 
Percolate. 
First  Portion. 
I 
Second  Portion.  ! 
Third  Portion. 
U.S.P. 
Differ- 
ences. 
Grammes. 
Acetic 
Acid. 
Differ- 
ences. 
Grammes. 
U.S.P. 
Differ- 
ences. 
Grammes. 
Acetic 
Acid. 
Differ-  I 
ences. 
Grammes. 
U  S  P 
Differ- 
Grammes. 
Acetic 
Acid. 
Differ- 
ences. 
Grammes. 
— 
ist  fraction  
3 '34 
603 
3*69 
7-16 
5-89 
7-42 
2d     "        ...  ... 
3-66 
577 
4-48 
7-26 
6-07 
7-68 
3d  "   
3-22 
5*oi 
4*53 
6-48 
57o 
6-69 
4th  "   
2"69 
4-42 
4-46 
6x3 
5'54 
57i 
5th  "   
2  "09 
3-47 
3"9i 
4-83 
478 
5"37 
6th  "   
2-23 
2-84 
4-29 
4'22 
472 
4-86 
7th  "   
179 
2-I7 
3"i9 
2*91 
3'5° 
4"  26 
8th  "   
1  "33 
1  "95 
2*40 
2'02 
3-06 
3-00 
gth     "  ...... 
1-17 
I"2I 
1  "57 
1 '44 
2'33 
1*97 
ioth  "   
I'I2 
•85 
1:43 
1.' 18 
1-89 
1-52 
nth  "   
•  77 
72 
"94 
74 
1*69 
I '02 
12th  "   
•51 
•40 
'94 
•72 
1-52 
•86 
X3th  " 
•37 
•06 
•70 
*53 
ri.6 
•62 
14th  "   
•27 
•18 
•84 
"37 
i-oo 
•72 
15th      "  ,   
.  "15 
•09 
•36 
•33 
79 
•33 
16th  "  
•19 
•13 
•33 
•26 
•76 
"43 
17th  "   
•11 
•05 
•34 
•29 
•65 
•27 
18th  "   
•26 
•14 
•61 
•32  • 
19th  "   
•18 
•00 
'47 
"ii 
20th      "  ...... 
•33 
•06 
The  next  table  deals  with  the  percolates  in  groups  of  five  frac- 
tions each,  the  measure  being  made  up  to  500  c.c.  as  described 
above,  and  these  larger  fractions  were  assayed  for  measure  and 
weight  of  fraction — for  total  extract — for  chloroform  extract  and 
for  total  of  mixed  alkaloids. 
The  most  significant  showing  of  this  table,  and  the  most  import- 
ant to  the  proposed  substitution,  is,  that  in  the  first  three  lines  of 
the  table  the  alcoholic  menstruum  has  extracted  85-3  per  cent,  of 
the  total  alkaloids  present,  while  the  acetic  acid  menstruum  has 
extracted  89-8  per  cent.    And  that  in  the  fifth  line  the  amount  of 
