A%i°rch,?899.rm*}      Leaves  of  Liatris  Odoratissima.  135 
Another  portion  of  the  -  said  aqueous  solution  was  agitated  with 
ether.  This  solvent,  upon  separation  and  evaporation,  left  a  crystal- 
line residue.    This  eventually  proved  to  be  coumarin. 
That  part  of  the  ether  extract  which  was  insoluble  in  hot  water, 
was  soluble  both  in  hot  alcohol  and  acetone.  The  alcoholic  solu- 
tion gave  a  black  precipitate  with  alcoholic  solution  of  ferric  chlo- 
ride, and  a  green  precipitate  with  alcoholic  solution  of  lead  acetate. 
When  a  third  portion  of  the  alcoholic  solution  was  poured  into 
water  a  precipitation  of  the  dissolved  substances  took  place. 
Besides  the  coumarin  and  the  resinous  matters  indicated  by  the  last 
three  tests,  the  ether  extract  contained  some  wax. 
Absolute  Alcohol  Extract. — This  extract  amounted  to  5-74  per 
cent,  of  the  leaves.  It  was  treated  with  hot  water,  which  dissolved 
everything,  but  a  little  chlorophyll.  The  aqueous  solution  had  a 
very  slightly  acid  reaction.  Fehling's  solution  applied  to  it,  failed 
to  show  the  presence  of  glucose,  but  after  boiling  the  aqueous  solu- 
tion with  acid,  this  reagent  was  reduced  to  an  extent  indicating  0*43 
per  cent,  of  cane  sugar.  The  aqueous  solution  produced  a  green 
color  with  ferric  chloride.  The  extract  contained  no  inorganic 
matter. 
Water  Extract. — The  organic  solids  of  the  cold  water  extract  of 
the  leaves  represented  42  96  per  cent,  of  their  weight.  Alcohol 
added  to  the  solution  of  the  water  extract  in  the  proportion  of  five 
volumes  of  the  former  to  one  of  the  latter  precipitated  17-28  per 
cent,  of  mucilage  and  albuminous  matters.  4-75  per  cent,  of  glu- 
cose was  indicated  in  this  extract ;  cane  sugar  was  absent.  Tannin 
too  was  absent,  as  was  also  shown  by  tests  applied  to  a  cold  water 
infusion  of  a  fresh  portion  of  the  leaves. 
Alkaline  Water  Extract. — When  the  residue  of  the  leaves  from 
the  treatments  with  the  foregoing  solvents  was  treated  with  water 
made  distinctly  alkaline  with  sodium  hydrate,  8-44  per  cent,  of 
organic  extract  was  removed.  This  amount  included  2  per  cent,  of 
mucilaginous  matter,  which  was  precipitated  when  the  solution  was 
acidified  with  acetic  acid,  and  then  mixed  with  five  times  its  volume 
of  alcohol. 
Acidulated  Water  Extract. — Water  acidulated  with  hydrochloric 
acid  was  next  applied  to  the  residue  of  the  leaves.  This  solvent 
dissolved  2*59  per  cent,  of  organic  matter.  Not  more  than  traces 
of  the  dissolved  substances  were  precipitated  by  making  the  solu- 
