Am.  Jour.  Pharm. 
April,  1899. 
Bark  of  Pinus  Echinata. 
167 
dissolved  it.  Upon  the  addition  of  acetic  acid  it  was  precipitated. 
Ammonium  hydrate  dissolved  this  precipitate.  It  was  again  pre- 
cipitated with  acetic  acid.  This  precipitate  reduced  nitric  acid.  It 
was  found  to  be  phlobapheries. 
The  portion  of  the  redeposited  material  above  spoken  of,  which 
was  insoluble  in  potassium  hydrate  was  of  a  pale  yellow  color  and 
rather  flocculent  character.  It  was  tested  for  nitrogen  by  Lassaigne's 
method.  No  nitrogen  was  found,  indicating  the  absence  of  albu- 
minous matter. 
A  portion  of  the  clear  alcoholic  solution  was  evaporated  to  dry- 
ness and  the  residue  treated  with  nitric  acid  which  was  reduced. 
Alcoholic  ferric  chloride  gave  a  green  color,  and  lead  acetate  a 
brown  precipitate,  all  of  which  indicate  phlobaphenes. 
Absolute  Alcohol  Extract. — This  extract  was  of  a  chocolate  color, 
had  a  nauseous  odor,  and  was  of  a  porous  character.  It  comprised 
9  03  per  cent,  of  the  bark.  It  was  first  treated  with  water  and 
filtered. 
The  soluble  matter  of  this  filtrate  comprised  3-16  per  cent,  of 
the  bark.  It  was  made  up  to  a  definite  volume  and  examined  as 
follows : 
An  aliquot  portion  was  completely  precipitated  with  basic  lead 
acetate  and  filtered.  The  lead  contained  in  the  filtrate  was  then 
precipitated  with  hydrogen  sulphide  and  filtered.  The  resulting 
filtrate  was  then  heated  on  water-bath  until  the  vapor  emanating 
therefrom  no  longer  acted  on  lead  acetate  paper.  It  was  again 
filtered  and  divided  into  two  equal  portions.  The  first  portion  was 
made  alkaline  and  treated  with  Fehling's  solution.  The  resulting 
precipitate  of  cuprous  oxide  showed  the  presence  of  glucose.  This 
precipitate  was  collected  on  a  filter  and  washed.  It  was  then  ignited 
and  weighed.  The  percentage  of  glucose  was  then  calculated  and 
found  to  be  075  per  cent,  of  the  bark. 
The  second  portion  of  this  prepared  solution  was  boiled  with  a 
few  drops  of  sulphuric  acid.  .  It  was  then  made  alkaline  and  treated 
with  Fehling's  solution.  The  resulting  precipitate  of  cuprous  oxide 
was  collected,  washed  and  ignited  to  constant  weight.  This  precipi- 
tate was  heavier  than  the  precipitate  obtained  from  first  portion. 
This  increase  in  weight  was  due  to  inverted  sugar,  Upon  calcu- 
lating the  percentage,  it  was  found  to  be  0*13  per  cent,  of  the 
bark. 
