68 
Bark  of  Pinus  Iichinata. 
( Am.  Jour.  Pharna 
I      April,  18D9. 
In  another  aliquot  portion,  the  tannin  was  estimated  according  to 
the  gelatin  and  alum  method.  It  comprised  017  per  cent,  of  the 
bark. 
Another  aliquot  portion  was  shaken  out  with  ether  in  a  separa- 
tory  funnel.  The  ethereal  layer  was  evaporated  and  the  residue 
taken  up  in  water  and  the  following  tests  applied  : 
Lead  acetate   .  Slight  lemon-yellow  precipitate. 
Gelatin  Slight  coloration. 
Lime  water   .  .  .  Reddish-brown  precipitate. 
Bromine  water  Yellow  precipitate. 
Ferrous  sulphate  crystal  No  change. 
Ferric  chloride  Slight  green  color. 
Sodium  carbonate  did  not  develop  a  red  color.  These  tests 
proved  the  absence  of  proto-catechuic  acid.  The  reactions  obtained 
were  due  to  tannin. 
The  remainder  of  this  solution  was  shaken  out  with  ether,  petro- 
leum ether  and  chloroform  in  a  separatory  funnel.  These  solvents 
were  applied  one  at  a  time,  and  in  each  case  the  solvent  was  drawn 
off  before  the  next  was  put  on.  The  various  solutions  were  evapo- 
rated and  their  residues  taken  up  in  water.  This  solution  was  tested 
for  glucosides,  but  none  was  found.  After  the  above  solution  had 
been  shaken  out  with  ether,  petroleum  ether  and  chloroform,  it 
was  made  alkaline  and  again  shaken  out  with  ether.  The  ethereal 
layer  was  evaporated  and  the  residue  taken  up  in  acidulated  water. 
This  solution  was  then  tested  for  alkaloids  with  Mayer's  reagent, 
phospho-tungstic  acid,  gallotannic  acid,  picric  acid,  platinic  chloride, 
gold  chloride  and  potassium  tri-iodide,  none  of  which  revealed  the 
presence  of  alkaloids. 
The  part  of  the  extract  insoluble  in  water  amounted  to  5*87  per 
cent,  of  the  bark.  It  was  entirely  soluble  in  dilute  ammonia  water. 
This  solution  was  precipitated  with  acetic  acid  and  the  resulting  pre- 
cipitate was  tested  with  nitric  acid  and  alkalies.  It  proved  to  be 
phlobaphenes. 
Water  Extract, — Water  extracted  2- 1 8  per  cent,  of  organic  solids 
from  the  bark. 
An  aliquot  part  of  the  volume  was  mixed  with  five  times  its  vol- 
ume of  alcohol  and  allowed  to  stand.  The  resulting  precipitate  was 
collected  on  balanced  filter  and  dried  at  no°.  It  was  tested  for 
nitrogen  by  Lassaigne's  method,  but  none  was  found,  proving  it  to 
be  a  mucilaginous  substance.   It  comprised  C42  per  cent,  of  the  bark. 
